高脂饮食及罗格列酮对C57BL／6小鼠肝脏AGFmRNA表达的影响  

Effects of high-fat diet and rosiglitazone on AGF mRNA expression in liver of C57BL/6 mice

作　　者：黄琦[1] 徐焱成[1] 

机构地区：[1]武汉大学中南医院内分泌科,武汉430071

出　　处：《中国医师杂志》2012年第7期907-909,共3页Journal of Chinese Physician

摘　　要：目的探讨小鼠肝脏组织血管生成素相关生长因子（Angiopietin—related growth factor，AGF）mRNA表达水平的变化与胰岛素抵抗的关系。方法将雄性C57BIM6小鼠以高脂饮食喂养10周，建立胰岛素抵抗模型，再以罗格列酮灌胃8周；实验结束时以口服糖耐量试验、HOMA评分评价实验小鼠血糖、胰岛素抵抗水平，用RT-PCR技术检测这三组小鼠肝脏组织AGFmRNA的表达，并进行统计学分析。结果高脂饮食组小鼠肝脏AGFmRNA的表达低于对照组，差异具有统计学意义（P〈0．05），罗格列酮组灌胃后小鼠肝脏AGFmRNA的表达增加（P〈0．01）；相关分析显示小鼠肝脏AGFmRNA表达与血浆胰岛素水平呈负相关（r=-0．516，P〈0．05）。结论高脂饮食诱导小鼠血浆葡萄糖及胰岛素水平增高，肝脏AGFmRNA表达降低，当胰岛素抵抗程度得到改善时，AGF的表达随之增加。Objective To study the correlation between diabetes and the new discovered angiopie- tin-related growth factor （AGF） that participates in energy metabolism. Methods A total of 24 male C57BL/6 mice were divided into three groups： controlled group; fed with high-fat diet（ HFD）; fed with high-fat diet for 10 weeks and then treated with rosiglitazone （ HFD ＋ RSG） ; The relative level of AGF mR- NA expression in livers was measured by determining a ratio of PCR products of AGF to that of ^-actin gene. Oral glucose tolerance test, and insulin resistance index （HOMA-IR） were used to measure blood glucose level and insulin sensitivity. Results Compared with the controls, the expression of AGF mRNA in liver decreased significantly in HFD group （ P 〈 0. 05 ） , and increased significantly in HFD ＋ RSG group （ P 〈0. 01 ）. A negative correlation was found between AGF mRNA expression in mice livers and HOMA score （ r = -0. 516, P 〈 0. 05）. Conclusions AGF as a new hepatocyte derived circulating factor counteracts obesity and is related to insulin resistance.

关 键 词：膳食脂肪类/副作用 噻唑烷二酮类/药理学 肝/代谢 血管生成素相关生长因子/ 代谢/遗传学 小鼠 

分 类 号：R587.1[医药卫生—内分泌]