DCE-MRI评价恩度联合阿瓦斯汀抗肿瘤血管生成疗效  

Antiangiogenic effect of Endostar combined with Avastin:a dynamic contrast-enhanced magnetic resonance imaging study

作　　者：田迎[1] 史红媛[1] 袁彩云[1] 罗松[1] 王建东[2] 卢光明[1] 

机构地区：[1]南京军区南京总医院医学影像科,南京210002 [2]南京军区南京总医院病理科,南京210002

出　　处：《放射学实践》2012年第8期820-824,共5页Radiologic Practice

摘　　要：目的:利用动态增强磁共振成像技术(DCE-MRI)评价恩度单独及恩度联合阿瓦斯汀抑制肿瘤血管生成的疗效。方法:将18只人肺腺癌细胞系A549皮下接种裸鼠,随机分成对照组(n=6)、恩度单独给药组(n=6)和恩度联合阿瓦斯汀给药组(n=6)三组,DCE-MRI结合病理学方法评价肿瘤血管生成及其渗透性。结果:给药7天后,对照组肿瘤体积为(0.85±0.25)cm3,恩度单独给药组为(0.37±0.17)cm3,联合给药组为(0.16±0.03)cm3(F=24.455,P=0.000);对照组血管功能参数Ktrans、Kep、Ve及iAUC分别为(0.11±0.03)min-1、(0.67±0.54)min-1、(0.35±0.12)、(13.28±4.25),恩度单独给药组分别为(0.06±0.01)min-1、(0.40±0.25)min-1、(0.31±0.09)、(7.33±3.93),联合给药组分别为(0.04±0.02)min-1、(0.27±0.12)min-1、(0.36±0.19)、(4.29±3.53),给药组Ktrans及iAUC值较对照组明显减小,差异均具有统计学意义(F=15.257,P=0.000;F=8.197,P=0.004);对照组微血管密度(MVD)为(14.24±3.38)条/高倍视野,恩度给药组为(8.88±1.86)条/高倍视野,联合给药组为(6.47±1.78)条/高倍视野,差异具有统计学意义(F=41.305,P=0.000);免疫组化结果显示给药组VEGF呈低表达,对照组呈高表达。结论:DCE-MRI定量参数对评价肿瘤血管生成与病理学结果具有一致性,DCE-MRI具有实时动态、无创等优点,能早期监测肿瘤生长、评价抗肿瘤血管生成药物的疗效。Objective;To evaluate antiangiogenic effect of endostar or endostar combined with avastin in a xenograft model of human lung cancer using dynamic contrast-enhanced magnetic resonance imaging （DCE-MRI）. Methods.. 18 nude mice,which were inoculated subcutaneously with human lung adenocarcinoma cells A549, were divided into three groups, control group （n： 6）, endostar group （n： 6） and combination of endostar and avastin （n： 6）. Tumor angiogenesis and vascular permeability were assessed with DCE-MRI and pathology methods. Results 7 days after antiangiogenic treatment, tumor volume among the control group, the endostar group and the combination treatment group were （0.85 ± 0.25）cm3 , （0.37±0. 17） cm3 and （0. 16±0. 03） cm3 , respectively. Ktrans, Kep, Ve and iAUC among three groups were （0. 11 ± 0.03）min-1 ,（0.67±0.54）min-1 ,（0.35±0.12） and （13.28±4.25）, （0.06±0.01）min-1 ,（0.40±0.25）min-1 ,（0.31± 0.09） and （7.33±.93）,（0.04±0.02）min-1 ,（0.27±0.12）min-1 ,（0.36±0.19） and （4.29±.53）. Significant decreases were reached in Ktrans,Kep and iAUC after antiangiogenic treatment, （F= 15. 257, P=0. 000 and F： 8. 197 ,P： 0. 004 respectively）. Microvessel density （MVD） among the control group, the endostar group and the combination treatment group were （14.24±3.38）item/hpf, （8.88± 1.86）item/hpf and （6.47 ±1.78）item/hpf, respectively. A significant de- crease in MVD was noted after antiangiogenic treatment （F= 41. 305, P= 0. 000）. There were statistically significant differ- ences in VEGF expression among three groups. A significant decrease in VEGF was reached after antiangiogenic treatment. Conelusion：DCE-MRI is a non-invasive functional imaging technique that permits indirect measurement of tumor angiogene- sis. It may therefore be suitable for monitoring the antiangiogenic effects of endostar combined with avastin.

关 键 词：磁共振成像 恩度 阿瓦斯汀 抗肿瘤血管生成 

分 类 号：R445.2[医药卫生—影像医学与核医学] R73-36[医药卫生—诊断学]