聚氨基苯甲酸修饰电极的制备及其在阿卡波糖含量测定中的应用  

Preparation of Poly-aminobenzoic Acid Modified Glassy Carbon Electrode and Application of Its in Content Determination of Acarbose

作　　者：靳贵英 肖树雄 

机构地区：[1]广东省食品药品检验所,广州510180

出　　处：《中国药房》2012年第29期2746-2748,共3页China Pharmacy

基　　金：广东省药检系统课题(ZA20101509)

摘　　要：目的:建立测定阿卡波糖含量的新方法。方法:采用电化学分析法。先通过循环伏安法将氨基苯甲酸电聚合在电极表面形成聚氨基苯甲酸,再利用铁氰化钾作为探针分子,以其在修饰电极上产生的氧化峰电流,采用方波伏安法进行阿卡波糖的含量测定,电位窗口为-0.1～+0.6V。结果:阿卡波糖检测浓度线性范围为0.10020～2.5050μg·mL-(1r=0.9998),平均回收率为96.33%,RSD=2.1%(n=4)。结论:该方法快速、简便,可用于片剂中阿卡波糖的含量测定。OBJECTIVE： To establish the method for the content determination of acarbose. METHODS： By electro-chemical analysis, a poly-aminobenzoic acid modified glassy carbon electrode was prepared by electropolymerization. Using potassium ferri-cyanide as probe molecular and oxidation peak current of modified electrode, SWV was used to determine the content of acarbose with potential window of -0.1-＋0.6 V. RESULTS： The linear range of acarbose was 0.100 20-2.505 0 μg·mL^-1 （r=0.999 8） with an average recovery of 96.33% （RSD=2.1%, n=4）. CONCLUSION： The method is rapid, simple and suitable for the con-tent determination of acarbose.

关 键 词：聚氨基苯甲酸修饰电极 电化学分析法 循环伏安法 方波伏安法 阿卡波糖 铁氰化钾 含量测定 

分 类 号：R927.2[医药卫生—药学] R977.15