巫山淫羊藿离体胚培养的研究  

In vitro embryo culture of Epimedium wushanense

作　　者：周海琴[1] 朱国胜 郭巧生[1] 刘作易 周宁 

机构地区：[1]南京农业大学中药材研究所,江苏南京210095 [2]贵州省现代中药材研究所,贵州贵阳550006 [3]贵州同济堂制药有限公司,贵州贵阳550002

出　　处：《中国中药杂志》2012年第14期2046-2051,共6页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2009BAI74B02);贵州省科技计划项目(黔科合院所创能[2010]4002);中央补助地方科技基础条件专项基金项目(黔科条中补地[2010]4002号)

摘　　要：目的:研究药用植物巫山淫羊藿组织培养技术,为工厂化育苗提供科学根据。方法:以巫山淫羊藿的种胚为外植体,采用MS培养基,附加不同浓度2,4-D,6-BA,IBA,NAA进行正交试验。结果:诱导愈伤组织的最优培养基为:MS+2,4-D2 mg.L-1+IBA 2 mg.L-1+NAA 0.5 mg.L-1;愈伤组织分化的最优培养基为:MS+6-BA 1 mg.L-1+NAA 0.5 mg.L-1+IBA 1 mg.L-1;诱导芽的最优培养基为:MS+IBA 2 mg.L-1+6-BA 0.5 mg.L-1;芽增殖的最佳培养基为:MS+6-BA 1.0 mg.L-1+NAA 0.5 mg.L-1。结论:通过诱导愈伤组织途径和丛生芽途径,建立了巫山淫羊藿种胚外植体诱导和培养方法,达到快速繁殖的目的。Objective： To study the in vitro embryo culture of Epimedium wushanense and provide scientific basis for large scale production of tissue culture. Method： Cullus and buds were induced from embryo of E. wushanense on a MS medium supplemented with different 2,4-D,6-BA, NAA, IBA. Result： The optimal compositions of medium that induced callus and buds from embryo were the MS medium supplemented with 2,4-D 2 mg · L^ -1 ,IBA 2 mg· L^-1 and NAA O. 5 mg · L^-1 and the MS medium supplemented with IBA 2 mg· L^-1 and 6-BA 0. 5 mg· L^-1, respectively. The optimum medium for callus differentiation was MS ＋ 6-BA 1 mg · L^-1 ＋ NAA 0. 5 mg · L^-1 ＋ IBA 1 mg · L^-1 , and MS ＋ 6-BA 1, 0 mg · L^-1 ＋ NAA O. 5 mg· L^-1 for shoots proliferation. Conclusion： Using embryo as explants, the method of induction and culture of E. wushanense was established by the callus and buds, and the embryo of E. wushanense can be quickly propagated.

关 键 词：巫山淫羊藿 种胚 组织培养 植株再生 

分 类 号：S567.239[农业科学—中草药栽培]