凋膜止崩液对无排卵性功血大鼠子宫内膜组织ER、ERα及ERβ表达的影响  

Effects of Diaomozhibeng Liquid on the Expression of ER,ERα and ERβ of Uteri Endometrial Tissue in Rats with Endometrial Hyperplasia

作　　者：贺丰杰[1] 李小宁[1] 张倩[1] 白俊[1] 

机构地区：[1]陕西中医学院,咸阳712046

出　　处：《天津医药》2012年第8期793-795,共3页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30873354);陕西省"13115"科技创新工程重大科技专项计划项目(项目编号:2008ZDKG-81)

摘　　要：目的:探讨凋膜止崩液宫腔靶向给药治疗无排卵性功能失调性子宫出血(功血)子宫内膜增生症的可能机制。方法:选取健康成年SD雌性大鼠48只,分为空白组、模型对照组、实验小剂量组和实验大剂量组,建立无排卵性功血子宫内膜增生症模型。凋膜止崩液宫腔内给药后3d处死,留取子宫标本,采用免疫组化技术检测各组大鼠子宫内膜组织中雌激素受体(ER)、ERα及ERβ的表达水平。结果:模型对照组ER、ERα、ERβ的表达高于空白组,实验大、小剂量组ER、ERα、ERβ的表达低于模型对照组,差异均有统计学意义(均P<0.001),实验大剂量组ER、ERα和ERβ水平低于实验小剂量组,差异有统计学意义(P<0.01)。结论:凋膜止崩液通过下调ER、ERα及ERβ阻断了雌激素发挥效应的通路,抑制子宫内膜的持续性增生从而治疗子宫内膜增生症。Objective： To explore the role and the possible mechanism ot Diaomozhibeng liquid in rats with the endome- trial hyperplasia （EH）. Methods： Forty-eight SD rats were divided into four groups including control group, model control group, the experimental low-dose group and the experimental high-dose group. The rat model of endometrial hyperplasia was induced with estrogen load. The Diaomozhibeng liquid was administrated in uterine cavity in rats. The expression levels of es- trogen receptor （ER）, ERα and ERβ were checked by immunohistochemistry. Results： Compared with control group, expres- sions of ER, ERa and ERβ increased in rats of the model group （P 〈 0.001）. However, after treatment with Diaomozhibeng liq- uid, the expression of ER, ERα and ERβ significantly decreased （P 〈 0.001）. Expression levels of ER, ERα and ERβ were significantly lower in high-dose experimental group than those of low-dose experimental group （P 〈 0.05 or P 〈 0.01）. Con- clusion： Diaomozhibeng liquid plays a role in endometrial hyperplasia by accelerating the ecclasis of uterine endometrial. One of mechanisms is maybe related to inhibiting the expression of ER, ERα and ERβ

关 键 词：功能性子宫出血 子宫内膜增生 受体 雌激素 雌激素受体α 雌激素受体Β 凋膜止崩液 

分 类 号：R169.41[医药卫生—公共卫生与预防医学]