红芪多糖对S_(180)瘤细胞化疗协同增效作用的蛋白质组学分析  

Proteomics Analysis of the Synergy Effect of Radix Hedysari Polysaccharides Combined with Chemotherapy on S_(180) Tumor Cells

作　　者：卫东锋[1] 赵春燕[2] 程卫东[1,3] 韦艳霞[1] 张李峰[4] 苏刚[4] 

机构地区：[1]兰州大学中西医结合研究所,甘肃兰州730000 [2]兰州大学药学院,甘肃兰州730000 [3]北京师范大学,北京100009 [4]兰州大学基础医学院,甘肃兰州730000

出　　处：《中药材》2012年第5期749-756,共8页Journal of Chinese Medicinal Materials

基　　金：中国博士后科学基金特别资助(201003073);中国博士后科学基金资助(20090450017);教育部高等学校博士点基金项目(200807330006)

摘　　要：目的:分析红芪多糖(Radix Hedysari polysaccharides,HPS)对S180瘤细胞化疗协同增效作用的差异蛋白质。方法:以环磷酰胺(Cyclophsphamide,Cy)和HPS联合Cy治疗荷瘤小鼠后提取瘤细胞总蛋白,用双向电泳对蛋白质进行分离,使用PDQuest 8.0软件对双向电泳图谱进行差异分析,通过质谱技术鉴定差异明显的蛋白点,并用蛋白免疫印迹方法对其中1个蛋白质进行验证。结果:HPS联合Cy组中有24个蛋白点发生明显改变,经质谱和Mascot软件分析,成功鉴定了5个蛋白质,分别为热休克蛋白84(HSP90β)、载脂蛋白A、白蛋白、热休克蛋白β1(HSP27)、未命名蛋白,其中1个高表达,4个低表达。蛋白印迹验证与蛋白质组结果一致。结论:通过蛋白质组学技术,发现了HPS对S180小鼠瘤细胞化疗增效作用的靶标蛋白,这些蛋白质涉及能量代谢、氧化应激反应和凋亡信号转导。Objective：To analyze the differentially expressed proteins of the synergy effect of Radix Hedysari polysaccharides （HPS）combined with chemotherapy（Cy） on S180 tumor cells. Methods：The total proteins extracted from the HPS combined with Cy treated S180 cells in tumor-bearing mice were separated by two dimentional gel electrophoresis （2-DE）and compared with those from Cy treated S180 cells using PDQuest 8.0 software. Mass spectrometry was applied to identify the differentially expressed proteins. Western blot was used to determine the differential expression of one protein. Results ：Twenty-four differentially-expressed proteins in HPS group were discovered. The five differential expressed proteins among twenty-four proteins were later identified by mass spectrometry and Mas-cot software as heat-shock protein hsp84 （HSP90β）, apolipoprotein A, albumin, heat shock protein beta-1 （HSP27）and unnamed protein product, including one up regulated and four down regulated expressed proteins respectively. Results from Western blot manifested the same trend as from proteomies analysis. Conclusion：Proteomics technique can be used to discover target proteins associated with the synergy effect of HPS combined with Cy on S180 tumor cells, involving some important proteins related to energy metabolism, oxidative stress and apoptosis induction signal transduction.

关 键 词：红芪多糖 S180瘤细胞 蛋白质组学 增殖抑制相关蛋白 凋亡诱导相关蛋白 

分 类 号：R285.5[医药卫生—中药学]