携载PSMA单抗靶向前列腺癌的纳米级脂质微泡的制备及体外寻靶能力  

Preparation and in vitro targeting ability of PSMA-targeted nanoscale lipid microbubbles

作　　者：李浪[1] 郭燕丽[1] 张淼[1] 谭开彬 华兴[1] 

机构地区：[1]第三军医大学第一附属医院超声科,重庆400038 [2]第三军医大学第二附属医院超声科,重庆400038

出　　处：《中国医学影像技术》2012年第8期1449-1453,共5页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金(30970830);重庆市科委自然科学基金(cstc2012jjB10012)

摘　　要：目的制备携载PSMA单抗靶向人前列腺癌的纳米级脂质微泡,并观察其体外寻靶能力。方法利用静电吸附法制备携载PSMA单抗靶向前列腺癌的脂质纳米级微泡,检测其一般特性;免疫荧光法检测抗体与纳米级微泡的结合情况;用细胞免疫荧光分析鉴定PSMA的表达及其在细胞膜上的定位;普通光镜下观察靶向微泡对前列腺癌细胞的寻靶能力,同时以胃癌细胞作对照。结果携载PSMA单抗的靶向纳米级微泡分布均匀,平均粒径为(623.70±66.05)nm,免疫荧光法显示微泡表面可见绿色荧光,细胞免疫荧光检测前列腺癌细胞膜高表达PSMA,体外寻靶实验显示该靶向微泡可与人前列腺癌LNCAP及C4-2细胞牢固结合,而与胃癌MKN45细胞不结合。结论本实验成功制备的携载PSMA单抗靶向人前列腺癌的纳米级脂质微泡具有较强的体外寻靶能力,能特异性地与前列腺癌细胞结合。Objective To prepare prostate-specific membrane antigen （PSMA）-targeted nanoscale lipid microbubbles of human prostatic carcinoma, and to observe the targeting ability of the microbubbles in vitro. Methods To prepare targeted nanoscale lipid microbubbles, the human PSMA was attached to the surface of nanoscale lipid microbubbles by electrostatic attraction, and their general characteristics were detected. Immunofluorescence was used for identification of PSMA ex- pression and its positioning in the membrane. The combination of PSMA with nanoscale lipid microbubbles was proved by immunofluorescent assay. Light microscopy was used to observe the targeting function of the combined effection of the tar- geted nanoscale lipid microbubbles with human prostatic carcinoma cells in vitro, and human gastric carcinoma cells were served as control. Results PSMA-targeted nanoscale lipid microbubbles distributed evenly, with average particle size of （623.70 ± 66.05）nm. Immunofluorescence assay showed green fluorescence visible on the surface of the targeted nanoscale lipid microbubbles. The expression of PSMA in human prostatic carcinoma cells was high, and the targeted nanoscale lipid microbubbles surrounding human prostatic carcinoma LNCAP and C4-2 cells with solid combination were detected, but not surrounding gastric carcinoma MKN45. Conclusion Targeting prostatic carcinoma nanoscale lipid microbubbles are suc- cessfully prepared, which having strong capability in vitro to find the target and specifically combine with prostatic carcino- ma cells.

关 键 词：纳米技术 微泡 前列腺肿瘤 前列腺特异性膜抗原 单克隆抗体 

分 类 号：R3[医药卫生—基础医学] R445.1