30株海洋绿藻的总脂含量和脂肪酸组成  

Fatty Acid Composition and Total Lipid Content of 30 Strains of Marine Green Algae

作　　者：曹春晖[1] 孙世春[1] 麦康森[1] 梁英[1] 

机构地区：[1]青岛海洋大学水产养殖实验室,青岛266003

出　　处：《青岛海洋大学学报（自然科学版）》2000年第3期428-434,共7页Journal of Ocean University of Qingdao

基　　金：国家海洋863高新技术发展计划项目!( 81 9-0 2 -0 1 )资助

摘　　要：对 11属 (小球藻属、绿囊藻属、微绿球藻属、海绿球藻属、卵胞藻属、原球藻属、咸胞藻属、杜氏藻属、裂丝藻属、塔胞藻属和衣藻属 )的 30株海洋绿藻进行特定条件下的一次性培养 ,在指数生长末期收获 ,进行了总脂含量和脂肪酸组成的分析。 2 1株海洋绿藻的总脂含量超过干重的10 % ,达 11.6 1%～ 34.4 9% ,其它 9株在 4 .2 5%～ 9.4 8%之间 ,绿藻的 16碳和 18碳脂肪酸最为丰富 ,有着含量较高的 16∶ 0、16∶ (n- 3)、18∶ 2 (n- 6 )和 18∶ 3(n- 3)脂肪酸。两株小球藻 (C95,C97)的2 0∶ 5(n- 3)脂肪酸含量较高 ,分别为 2 0 .8%和 2 6 .1%。另一株小球藻 (C10 2 )和两株裂丝藻 (C19和C2 0 ) EPA含量居中 ,分别为 8.0 % ,6 .0 %和 8.1%。其它藻株一般只含有少量的 2 0∶ 5(n- 3)和 2 2∶ 6 (n- 3)或不含 2 2∶ 6 (n- 3)Total lipid content and fatty acid composition of 30 strains of 11 genera (Chlorella, Chlorosarcinopsis,Nannochloris, Halochlorococcum.Oocytis, Protococcus, Brachiomonas, Dunaliella, Stichococcus, Pyramimonas and Chlamydomonas) of marine green algae were studied. The microalgae were cultured under defined conditions and harvested during later log phase. Total lipid contents and fatty acids were determined and analysed. The results suggest that the total lipid contents of marine green algae were more than 10% of dry weight (11.61%～34.49%)except 9 strains (4.52%～ 9.48% ).High levels of 20∶5(n 3) were present in two strains of Chlorella (C95,C97)( 20.8% and 26.1% of the total fatty acids), another Chlorella(C102) and two strains of Stichococcus (C19 and C20) had middle levels of EPA(8.0%,6.0% and 8.1%).Little 20∶5(n 3) and 22∶6(n 3) FA were detected in the other strains. The major fatty acids in green algae were 16∶0,16∶1(n 7),16∶4(n 3),18∶1(n 9),18∶2(n 6) and 18∶3(n 3)

关 键 词：海洋绿藻 总脂 脂肪酸 

分 类 号：Q949.210.6[生物学—植物学]