高效液相色谱法测定乙肝康颗粒中芍药苷、大黄素、丹参酮ⅡA的含量  

Simultaneous determination of paeoniflorin,emodin and tanshinoneⅡA in Yigankang Keli by HPLC

作　　者：刘彦 王宁[2] 

机构地区：[1]湖南溆浦县人民医院,湖南怀化419300 [2]怀化市食品药品检验所,湖南怀化418000

出　　处：《中南药学》2012年第7期512-515,共4页Central South Pharmacy

摘　　要：目的建立高效液相色谱法测定乙肝康中芍药苷、大黄素、丹参酮ⅡA的含量。方法色谱柱为ThermoC18column(250mm×4.6mm,5μm);流动相为甲醇-0.1%磷酸(30∶70);流速1.0 mL·min-1;测定波长230nm;柱温35℃;进样10μL。结果芍药苷、大黄素、丹参酮ⅡA的线性范围分别为0.010 45～0.125 4μg(r=0.999 3,n=6),0.010 21～0.122 5μg(r=0.999 0,n=6),0.010 56～0.126 7μg(r=0.999 2,n=6),平均回收率分别为99.0%、99.3%、99.1%,RSD分别为1.2%、1.1%、1.1%(n=9)。结论该方法操作简便,灵敏度高,重现性好,可用于乙肝康颗粒中芍药苷、大黄素、丹参酮ⅡA的含量测定。Objective To establish an HPLC method for the simultaneous determination of paeoniflorin,emodin and tanshinoneⅡA in Yigankang Keli.Methods The column was Thermo C18（250 mm ×4.6 mm,5 μm） with the mobile phase composed of methanol-0.1%phosphoric（30∶70）.The flow rate was 1.0 mL·min-1,the detection wavelength was 230 nm,the column temperature was 35 ℃ and the injection volume was 10 μL.Results The linear ranges of paeoniflorin,emodin and tanshinoneⅡA were 0.010 45-0.125 4（r=0.999 3,n=6）,0.010 21-0.122 5 μg（r=0.999 0,n=6）,and 0.010 56-0.126 7 μg（r=0.999 2,n=6）.The average recoveries of paeoniflorin,emodin and tanshinoneⅡA were 99.0%,99.3% and 99.1%.RSD was 1.2%,1.1% and 1.1%（n=9）.Conclusion The method is simple,accurate,sensitive and repeatable,which can be used to control the quality of Yigankang Keli.

关 键 词：高效液相色谱法 乙肝康颗粒 芍药苷 大黄素 丹参酮ⅡA 

分 类 号：R917[医药卫生—药物分析学] R927.2[医药卫生—药学]