PESV对K562细胞BCR/ABL融合基因及凋亡调控因子Bcl-2、Bad表达的影响  

The Effects of PESV on the Expression of BCR / ABL Fusion Gene and Bcl-2,Bad of Apoptosis Regulators on the K562 Cells

作　　者：于文俊[1] 杨文华[2] 杨向东[2] 史哲新[2] 王兴丽[2] 郝征[1] 张佳[1] 

机构地区：[1]天津中医药大学,天津300193 [2]天津中医药大学第一附属医院,天津300193

出　　处：《现代生物医学进展》2012年第21期4015-4018,共4页Progress in Modern Biomedicine

基　　金：教育部博士点基金(20091210110004)

摘　　要：目的:探讨PESV对K562细胞BCR/ABL融合基因及凋亡调控因子bcl-2和bad表达的影响。方法:将体外培养K562细胞,经PESV处理不同时间后,流式细胞术检测细胞凋亡率,荧光定量RT-PCR检测BCR/ABL、Bcl-2、Bad mRNA水平变化。结果:与对照组相比,PESV处理后K562细胞,凋亡率增加,BCR/ABL融合基因表达降低,抗凋亡相关基因Bcl-2 mRNA表达降低,促凋亡基因Bad mRNA表达增加。结论:PESV能降低降低K562细胞BCR/ABL融合基因的表达,可能通过调节Bcl-2和Bad表达,抑制K562细胞增殖,促进其凋亡。Objective： To investigate the PESV of K562 cells BCR / ABL fusion gene and apoptosis regulators bcl-2 and bad expression. Methods： K562 cells were cultured in vitro, by PESV for different times, the apoptosis rate by flow cytometry, fluorescence quantitative RT-PCR detection ofBCR / ABL, Bcl-2, Bad mRNA level changes. Results： Compared with the control group, PESV treated K562 cells, apoptosis increased, BCR / ABL fusion gene reduced expression, anti-apoptotic gene Bcl-2 mRNA expression decreased, pro-apoptotic gene Bad mRNA expression. Conclusion： PESV reduced in K562 cells can reduce the BCR / ABL fusion gene, may regulate the expression of Bcl-2 and Bad, inhibit proliferation ofK562 cells and promote their apoptosis.

关 键 词：PESV K562细胞系:凋亡 BCR/ABL BCL-2 BAD 

分 类 号：Q75[生物学—分子生物学] Q78