热休克蛋白70-hom基因多态性与儿童过敏性紫癜的相关性  

HSP70-hom polymorphism and susceptibility to childhood Henoch-Schonlein purpura

作　　者：徐锦雯[1] 赵丽萍[1] 朱春华[2] 韩媛[2] 张爱华[2] 黄松明[2] 

机构地区：[1]南京医科大学附属无锡人民医院儿科,江苏无锡214023 [2]南京医科大学附属南京儿童医院儿肾内科,江苏南京210008

出　　处：《南京医科大学学报（自然科学版）》2012年第8期1075-1078,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京市医学重点发展项目(ZKX08007)

摘　　要：目的:探讨热休克蛋白70-hom(heat shock protein 70-hom,HSP70-hom)基因多态性与儿童过敏性紫癜发病的相关性。方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测131例过敏性紫癜患儿和66例正常儿童的HSP70-hom基因的+2437位核苷酸的NcoⅠ限制性内切酶位点的多态性。结果:HSP70-hom各基因型频率在过敏性紫癜组中分别为1.5%(TT)、34.4%(TC)和64.1%(CC),在对照组中分别为19.7%(TT)、19.7%(TC)和60.6%(CC),两组之间基因型分布频率的差异具有统计学意义(χ2=22.318,P<0.05);T和C等位基因频率在过敏性紫癜组中分别为18.7%(T)和81.3%(C),在对照组中为29.5%(T)和70.5%(C),差别亦具有统计学意义(χ2=5.949,P<0.05)。与携带TT基因型者比较,携带至少1个C等位基因(即TC和CC基因型)的个体患过敏性紫癜的风险增加18.83倍(95%CI:3.95～89.75)。过敏性紫癜非肾脏损害组与肾损害组TT、TC、CC基因型频率分别为2.4%、32.1%、65.5%和0%、38.3%、61.7%,差异不具有统计学意义(χ2=0.379,P>0.05)。结论:HSP70-hom+2437T/C基因多态性是儿童过敏性紫癜发病的遗传易感因素,但与肾脏损害发生无显著相关性。Objective：To investigate the potential involvement of gene variants of heat shock protein 70-hom（HSP70-hom） in childhood Henoch-Schonlein purpura.Methods：Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was used to analyze the polymorphisms of HSP70-hom ＋2437T/C in 131 children with Henoch-Schonlein purpura and 66 health controls.Results：HSP70-hom gene frequencies in children with Henoch-Schonlein purpura were 1.5%（TT）,34.4%（TC）,64.1%（CC）,and 19.7%（TT）,19.7%（TC）,60.6%（CC） in health controls,the differences between two groups were statistically significant.In addition,the T,C allele frequencies were 18.7%（T）,81.3%（C） in Henoch-Schonlein purpura group,and were 29.5%（T）,70.5%（C） in control groups,the differences were statistically significant,too.Children carrying at least one ＋2437C variant allele（TC＋CC genotype） had a significantly increased risk for childhood Henoch-Schonlein purpura compared with the wildtype genotype（＋2437TT）.However,the differences between children without renal involvement in Henoch-Schonlein purpura and children with renal complications did not reach statistical significance.Conclusion：The polymorphisms of HSP70-hom＋2437 T/C are genetic predisposing predictors for Henoch-Schonlein purpura in children,but has no relationship with renal complications

关 键 词：热休克蛋白70 过敏性紫癜 多态性 

分 类 号：R554.6[医药卫生—血液循环系统疾病]