水飞蓟素对急性肺损伤小鼠肺组织IL-1β、IL-6、趋化因子fractalkine表达的影响  

The effects of silymarin on IL-1β,IL-6,fractalkine gene expression in lung tissue rats with acute lung injury

作　　者：张晓鸣[1] 顾绍庆[2] 

机构地区：[1]江苏大学附属医院儿科,江苏镇江212000 [2]江苏大学附属人民医院儿科,江苏镇江212000

出　　处：《南京医科大学学报（自然科学版）》2012年第8期1083-1086,共4页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:探讨水飞蓟素(silymarin,SIL)对急性肺损伤小鼠肺组织中白细胞介素(interleukin,IL)-1β、IL-6、趋化因子fractalkine表达的影响与意义。方法:利用内毒素(lipopolysaccharide,LPS)气管内滴入制备小鼠急性肺损伤的模型;设立SIL治疗组(S组)、LPS组(L组)、生理盐水对照组(N组),S组在气管内滴入LPS前6、4、2 h以200 mg/kg水飞蓟素灌胃,分别在LPS处理后6、12、24 h取肺组织匀浆,用ELISA法分别测定各组IL-1β、IL-6、fractalkine蛋白水平。结果:在各时间点,S组肺组织中IL-1β、IL-6、fractalkine蛋白表达水平均显著低于L组。结论:SIL能有效抑制小鼠急性肺损伤时肺组织中IL-1β、IL-6、趋化因子fractalkine的表达。Objective：To investigate the effects of silymarin（SIL） on the expressions of IL-1β,IL-6,and fractalkine in lung tissue of rat with acute lung injury.Methods：A rat model of acute lung injury（ALI） was induced by lipopolysaccharide（LPS）.All rats were randomly divided into SIL group,LPS group and normal saline control group.SIL（200 mg/kg） was dropped into airway of rats in SIL group 6,4,and 2 h before treated with LPS.After LPS was given for 6,12 and 24 h,IL-1β,IL-6,chemotatic factor fractalkine protein levels in lung tissue homogenate were detected by ELISA.Results：At all time point,IL-1β,IL-6,fractalkine protein levels of SIL group were all significantly lower than those in LPS group（P 〈 0.05）.Conclusion：SIL could significantly inhibit the expression of IL-1β,IL-6,fractalkine in lung tissue of rat with acute lung injury.

关 键 词：水飞蓟素 急性肺损伤 白介素-1Β 白介素-6 FRACTALKINE 

分 类 号：R563[医药卫生—呼吸系统]