丙烯腈对小鼠生精细胞Bcl-2、Bax蛋白表达的影响  

Effect of acrylonitrile on the expression of Bcl-2 and Bax in spermatogenic cell of mice

作　　者：裴凌云[1] 马国燕[1] 金娜[1] 陈亚[1] 冯玉娟[1] 党瑜慧[1] 薛红丽[1] 李芝兰[1] 

机构地区：[1]兰州大学公共卫生学院儿少所,兰州730000

出　　处：《卫生研究》2012年第4期638-641,共4页Journal of Hygiene Research

基　　金：中央高校基本科研业务费专项资金项目(No.LZUJBKY-2010-140)

摘　　要：目的探讨丙烯腈(AN)暴露对小鼠睾丸生精细胞Bcl-2、Bax蛋白表达水平的影响。方法将250只成年健康SPF级昆明种雄性小鼠,按体重随机分为阴性对照组、3个AN染毒组和阳性对照组,阴性对照组用生理盐水,各染毒组分别以1.25、2.50、5.00mg/kg AN腹腔注射(注射剂量为0.01ml/g BW),每天1次,连续5天,阳性对照组注射环磷酰胺40mg/kg一次。分别于首日染毒后的第7、14、21、28和35天采用颈椎脱臼法处死小鼠,采用免疫组织化学法(SABC)检测生精细胞Bcl-2和Bax蛋白的表达。结果 5个观察时间点AN 2.50mg/kg组和21天AN 1.25mg/kg组Bcl-2平均光密度值均显著低于阴性对照组(P<0.05);除21天AN 1.25mg/kg组外,所有观察时间点AN各染毒组Bax平均光密度值均显著高于阴性对照组(P<0.05)。Bcl-2在中剂量组和阳性对照组各观察时间点降低幅度均较大;Bax在各剂量组的不同时间点均以14天时升高幅度最大。结论 AN可诱导生精细胞Bcl-2蛋白表达减弱,以AN 2.50mg/kg组最为显著;同时可诱导生精细胞Bax蛋白表达增强,以14天时变化幅度较明显。Objective To explore the effect of acrylonitrile exposure on the expression of Bcl-2 and Bax in mice sperrnatogenic cells. Methods Based on body weight, 250 SPF Kunming adult male mice were randomly divided into five grouPs： negative control group （normal saline 0. 01ml/g）, three AN exposure groups （intraperitoneal injection of 1.25, 2. 50 or 5.00 mg/kg of AN daily for 5 days, respectively）and positive control group （intraperitoneal injection of cyclophosphamide 40mg/kg）. Mice were killed in the 7th, 14th, 21 st, 28th and 35th day after the first exposure by cervical dislocation~ Immunohistochemical method （SABC） was used to detect the expression of Bcl- 2 and Bax protein in spermatogenic cells~ Results The average optical density values of Bcl-2 at five time points of the AN 2. 50 mg/kg group and the 21th day point of the AN 1.25mg/kg group were significantly lower than the negative control group （P 〈 0. 05 ） ~ Except the 21st day point of the AN 1.25 mg/kg group, the mean optical density values of Bax in all time points of AN exposure groups were significantly higher than the negative control group （P 〈 0. 05）. The decreased expression of Bcl-2 protein was most distinct in AN 2.50mg/kg group and the positive control group at all time points. The expression of Bax protein was significantly increased in all groups at the 14th day point. Conclusion The expression of Bcl-2 protein could be weakened in spermatogenic ceils induced by AN, especially in the AN 2.50 mg/kggroup; while the expression of Bax was enhanced, and the amplitude of change in the 14th day point was more obvious.

关 键 词：丙烯腈 生精细胞 BCL-2 BAX 

分 类 号：R994.6[医药卫生—毒理学] Q26[医药卫生—药学]