剑叶耳草挥发油的GC-MS分析  

GC-MS Analysis of Volatile Oil from Hedyotis lancea

作　　者：潘为高[1] 李勇[1] 朱小勇[1] 朱意麟[1] 李耀华[1] 罗彭[1] 

机构地区：[1]广西中医药大学,南宁530001

出　　处：《中国实验方剂学杂志》2012年第15期130-134,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西中医学院院级课题(P2009044)

摘　　要：目的:研究剑叶耳草挥发油的化学成分组成。方法:分别采用水蒸气蒸馏法(SD)和超临界CO2萃取法(SFE)提取制备两种挥发油;通过气质联用分析其化学成分组成,用面积归一化法确定各成分的相对百分含量。结果:水蒸气蒸馏法挥发油中分离出67个色谱峰,鉴定其中32个化合物,占挥发油总量的73.76%;超临界CO2萃取法挥发油中分离出82个色谱峰,鉴定其中45个化合物,占挥发油总量的61.62%。结论:剑叶耳草挥发油主要包括脂肪族含氧衍生物、芳香族含氧衍生物和萜含氧衍生物,但两种提取方法的挥发油成分组成和含量有差异。Objective：To study the chemical constituents of volatile oil from Hedyotis lancea.Method： Two kinds of volatile oil were separately prepared by steam distillation（SD） and supercritical CO2 extraction（SFE）.The constituents were separated and analyzed by gas chromatography-mass spectrometry with the area normalization method to determine the relative percentage content of each component.Result： Sixty-seven compounds were isolated and 32 compounds were identified by SD that composed about 73.76% of the total essential oil;and 82 compounds were isolated and 45 compounds were identified by SFE that composed about 61.62% of the total essential oil.Conclusion： The principal chemical constituents of the volatile oil from H.lancea are aliphatic derivatives,aromatic derivatives and terpene derivatives.There are some differences in the principal chemical constituents and content of the volatile oil extracted by SD and SFE.

关 键 词：剑叶耳草 挥发油 超临界萃取法 水蒸气蒸馏法 GC-MS 

分 类 号：R284.1[医药卫生—中药学]