山绿茶中Ilexgenin A对HepG2肿瘤细胞抑制作用的研究  

Experimental Study of the Inhibiting Effects of Ilexgenin A from Ilex Hainanensis on Tumor Cell HepG2

作　　者：李洪亮[1] 徐仙赟[2] 孙立波[3] 程齐来[1] 

机构地区：[1]赣南医学院药学院,江西赣州341000 [2]赣南医学院基础医学院,江西赣州341000 [3]青岛大学医学院附属医院,山东青岛266001

出　　处：《中国实验方剂学杂志》2012年第15期238-241,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江西省自然科学基金项目(2010GQY0010);江西省教育厅科技计划项目(GJJ11206)

摘　　要：目的:探讨3β,19α-二羟基乌苏-12-烯-24,28-二羧酸(IA)对肝癌细胞株HepG2细胞周期、凋亡的影响及其作用机制。方法:人肝癌细胞株HepG2以IA 20,40,60,80 g·L-1作用不同的时间后,分别使用四甲基偶氮唑蓝比色法(MTT),吖啶橙(AO)荧光染色法,免疫细胞化学SP法,流式细胞术检测HepG2细胞的增殖抑制情况,细胞周期和凋亡的形态学变化,以及细胞内凋亡相关蛋白Bcl-2,Bax表达的变化。结果:IA对HepG2细胞增殖有抑制作用,其作用随着药物浓度和作用时间的增加而增强。IA作用24 h后HepG2细胞周期的主要特点是大量细胞累积于G1期,进入S期的细胞减少,IA能够诱导HepG2细胞凋亡,IA(40,60,80 g·L-1)经过24 h作用后,细胞凋亡率分别为7.89%,8.37%,9.93%,AO染色观察到大量凋亡细胞,凋亡细胞染色减弱、荧光较暗、呈均匀一致的圆状或固缩状,细胞内Bcl-2的表达随着药物浓度增加逐渐减少;Bax蛋白的表达随着药物浓度增加逐渐增加。结论:IA能够抑制HepG2细胞的增殖,并使细胞大量累积于G1期,进入S期的细胞减少,通过影响凋亡蛋白表达诱导细胞凋亡。Objective：To investigate the proliferation inhibition and apoptosis in human hepatic cancer cell strain HepG2 by ilexgenin A and its mechanism.Method：HepG2 were treated by iexgenin A with different concentration and time.MTT assay,around totally orange（AO） fluorescence staining method,flow cytometry（FCM）,SP method of immunocytechemistry were used to determine the inhibiting effect on HepG2 cells,the cell cycle and apoptosis morphological changes and the expressions of Bcl-2,Bax.Result：Ilexgenin A inhibited the proliferation of HepG2 cells in dosage and time dependent manner.After HepG2 cells were treated by ilexgenin A for 24 hour,ilexgenin A induced a G1 cell cycle arrest and reduced the cell population in S phase and the apoptosis rate were 7.89%,8.37%,9.93%,respectively.With the increasing of the medicine density,the expression of Bcl-2 was down-regulated,while the expression of Bax up-regulated in the apoptosis.Conclusion：Ilexgenin A can inhibit proliferation of the HepG2 cells and induced a G1 cell cycle arrest and reduced the cell population in S phase.It also can induce apoptosis of human prostatic carcinoma through down-regulating the expressions of Bcl-2,up-regulating the expression of Bax.

关 键 词：3β 19α-二羟基乌苏-12-烯-24 28-二羧酸 HEPG2细胞 抑制作用 山绿茶 乌苏烷三萜 

分 类 号：R287[医药卫生—中药学]