抗肿瘤海洋放线菌LYG-1的筛选及分类鉴定(英文)  

Isolation and characterization of a marine actinomyces LYG-1 with antitumor activity

作　　者：邢莹莹[1] 陆园园[1] 马益华[1] 鲍维维[1] 奚涛[1] 

机构地区：[1]中国药科大学生命与技术学院,南京210009

出　　处：《中国药科大学学报》2012年第4期366-370,共5页Journal of China Pharmaceutical University

摘　　要：从连云港海域海泥中分离得到一株具有抗肿瘤活性海洋放线菌LYG-1,通过对其形态特征、培养特征、生理生化特性的研究以及16S rRNA基因序列分析,将海洋放线菌LYG-1归属为链霉菌属玫瑰孢链霉菌(Streptomyces roseos-porus)的新的海洋变种。利用MTT法对海洋放线菌LYG-1发酵产物的抗肿瘤活性进行了初步研究,结果表明放线菌LYG-1的发酵原液对HepG2、MCF-7、HCT116以及MDA-MB-231 4种肿瘤细胞具有良好的体外抗肿瘤活性。Marine actinomyces LYG-1 was isolated from marine mud flats in Lianyungang, China. Strain LYG-1 was identified using the methods of morphology, physiological and chemotaxonomic characterization and 16S rRNA gene sequence analysis. The results showed that strain LYG-1 was a marine variable species of Streptomyces roseosporus. The fermentation broth of strain LYG-1 exhibited conspicuous antitumor activity against HepG2, MCF7, HCTll6 and MDA-MB-231 cell lines, and the IC50 values were defined by MTT method respectively.

关 键 词：海洋放线菌 筛选及分类鉴定 玫瑰孢链霉菌 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]