不同品种水蛭抗凝抗血栓作用的比较  

Comparative studies on anti-thrombus and anti-coagulation effects of Hirudo of different species

作　　者：关世侠[1] 袁中文[1] 周郁斌[1] 张怡[1] 叶小玲[1] 胡波[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《中国医院药学杂志》2012年第14期1093-1096,共4页Chinese Journal of Hospital Pharmacy

基　　金：广东省科技计划项目(编号:2010A030100010)

摘　　要：目的:比较研究宽体金线蛭和菲牛蛭的体内外的抗凝、抗血栓作用。方法:采用凝血酶直接滴定法测定不同处理方法的2种水蛭体外抗凝血活性;采用胶原蛋白-肾上腺素诱导小鼠脑血栓,角叉菜胶诱导小鼠尾静脉血栓以及大鼠动-静脉旁路血栓形成的方法,观察不同处理方法的两种水蛭的抗血栓作用。采用毛细管法和剪尾法,观察不同处理方法的两种水蛭的抗凝血作用。结果:菲牛蛭鲜体和干品的体外抗凝活性远优于鲜体及干燥宽体金线蛭;鲜品菲牛蛭、干燥菲牛蛭以及经不同方法处理的宽体金线蛭均具有明显的体内抗凝抗血栓活性,其中以菲牛蛭鲜品的作用最强烈;煎煮后的菲牛蛭几乎无体内外生物活性。结论:菲牛蛭与宽体金线蛭的活性成分有很大区别,且菲牛蛭比目前广泛应用的宽体金线蛭具有更优的药效活性。OBJECTIVE To study and compare the anti-thrombus effects and anti-coagulation activities of Hirudinaria ma- nillensis and Whitmania pigra in vivo and in vitro. METHODS The in vitro anti-coagulation activities of the two species of leeches after different processes were evaluated by directly titration on thrombin. The cerebral thrombosis induced by intrave- nous injection with adrenalin-collagen, caudal vein thrombosis caused by carrageenin and thrombosis formed in arteriovenousshunt models were used to assess the anti thrombus effects of the two species of leeches. The in vitro anti-coagulation activities of the two species of leeches after different processes were evaluated by cutting-tail method and capillary method. RESULTS The anti-coagulation activity in vitro of Whitmania pigra was quite better than the fresh and dry Hirudinaria rnanillensis. Fresh and dry Hirudinaria manillensis and Whitrnania pigra after different processes had anti-thrombus activities in vivo, and fresh Hirudinaria manillensis had the highest activity while Hirudinaria manillensis after decoction hardly had any activity in vivo and in vitro. CONCLUSION The active constituent of Hirudinaria manillensis is dramaticlly different from Whitmania pigra, moreover, Hirudinaria manillensis has much more bioactivity than Whitmania pigra which is widely used recently.

关 键 词：菲牛蛭 宽体金线蛭 抗凝 抗血栓 

分 类 号：R282.74[医药卫生—中药学]