柑桔溃疡病拮抗芽孢菌株的筛选及其控病作用  

Screening of Antagonistic Spore Bacteria for Biocontrol against Xanthomonas axonopodis pv. citri

作　　者：易龙[1] 马冠华[2] 

机构地区：[1]赣南师范学院生命与环境科学学院,赣州341000 [2]西南大学植物保护学院

出　　处：《中国南方果树》2012年第4期5-7,17,共4页South China Fruits

基　　金：公益性行业(农业)科研专项经费(201003067-02-6;201003067-02-7);江西省科技支撑计划项目(2010BNB01402)资助

摘　　要：从贛州脐橙园脐橙根际土壤中获得192个细菌分离物,通过平板拮抗测试,筛选出对柑桔溃疡病菌Xanthomonas axonopodis pv.citri有较强拮抗作用的6个芽孢菌株,抑菌圈直径达12.0～25.0mm,其中菌株G32对柑桔溃疡病菌抑制效果最好,稳定性高;温室控病试验表明,菌株G32的控病效果最佳,接种溃疡病菌前喷施拮抗菌G32的预防效果达60.7%,接种溃疡病菌后喷施拮抗菌G32的治疗效果为53.2%;根据其形态特征、培养性状、生理生化特性及16SrDNA序列比对分析,将G32鉴定为枯草芽孢杆菌Bacillus subtilis。192 isolates were obtained from rhizosphere soil of citrus orchards in Ganzhou, Jiangxi province. It was found that 6 potential biocontrol spore bacteria showed good antagonistic effect on Xanthomonas axonopodis pv. citri, the pathogen of citrus bacterial canker, for showing inhibition zones of 12. 0 mm to 25.0 mm in diameter by pairing culture on NA plate. The bacterial strain G32 had the best inhibitory effect on the pathogen and the highest stability. The control rate of strain G32 reached 60. 7% and 53.2% respectively when sprayed prior to and after the inoculation of citrus canker bacteria under greenhouse conditions. Based on morphological, cultural, physiological and bio- chemical characteristics, as well as its 16S rDNA sequence, G32 was identified as Bacillus subtilis.

关 键 词：柑桔溃疡病 枯草芽孢杆菌 生物防治 

分 类 号：S436.661.19[农业科学—农业昆虫与害虫防治]