高效色谱-电感耦合等离子体质谱法分析海藻类海产品中砷的形态  

Analysis of arsenic speciation in algae by high performance chromatography coupled inductively coupled plasma-mass spectrometry

作　　者：李卫华[1] 刘玉海[1] 

机构地区：[1]江苏技术师范学院化学化工学院,常州213001

出　　处：《分析试验室》2012年第8期74-78,共5页Chinese Journal of Analysis Laboratory

基　　金：江苏省省高等学校自然科学基金课题(07KJD150044)资助

摘　　要：采用阴(Hamilton PRP-X100柱)阳(Dionex Ionpac CS-10柱)离子交换色谱-电感耦合等离子体质谱联用技术,分别以pH 10.3的20 mmol/L NH4HCO3和pH 2.0的5 mmol/L吡啶溶液为流动相,建立了As(Ⅲ)、As(Ⅴ)、一甲基砷酸(MMA)、二甲基砷酸(DMA)、砷甜菜碱AsB、砷糖PO4、砷糖OH、砷糖SO3、砷糖SO4砷形态的分析方法。采用微波消解法和超声溶剂提取法对不同海域10种紫菜和海带产品进行前处理,对As含量及其化学形态进行分析。实验表明,样品总砷的质量分数为1.7～38.7 mg/kg,样品萃取物中,As糖PO4和As糖OH为As的主要形态,其含量分别占可提取As的6.5%～67.7%和12.9%～86.2%,海带样品萃取物中还有As糖SO3和DMA被检测,其含量分别占可提取As的13.0%～52.1%和5.9%～17.4%。在紫菜和海带海藻类产品中,含As的化合物主要是毒性较低的有机砷。The separation of arsenic compounds,including of As（Ⅲ）,As（Ⅴ）,MMA,DMA,Arsenobetaine（AsB）,Arsenosugar PO4,Arsenosugar OH,Arsenosugar SO3,Arsenosugar SO4 were carried out in Hamilton PRP-X100 anion-exchanged column with a mobile phase of 20 mmol/L NH4HCO3（pH 10.3） and on a Dionex Ionpac CS-10 cation-exchanged column with a mobile phase of 5 mmol/L pyridinium（pH 2.0） using ICP-MS.Total content of As as well as its different species of ten types of red algae and brown algae in microwave-assisted digestion and ultrasonic solvent extraction were examined.The results showed that total arsenic concentration in algae samples was 1.7～38.7 mg/kg As（dry weight）.Main components in all of the algae samples were Arsenosugar PO4 and Arsenosugar OH（6.5%～67.7%,12.9%～86.2%）,while Arsenosugar SO3 and DMA were detected in all of the brown algae samples（13.0%～52.1%,5.9%～17.4%）.In contrast,no inorganic arsenic was detected in algae samples.Since the major share of arsenic components in algae is organic arsenic with a low toxiticy,we can conclude that arsenic in algae does not pose any risk to human health.

关 键 词：HPLC-MS 砷 形态 海藻类海产品 

分 类 号：O657.63[理学—分析化学]