同多酸和染料探针共振光散射法测定蛋白质  

Determination of protein using isopolyacid and dye as probes by resonance light scattering

作　　者：张婷[1] 冯素玲[1] 危彬[1] 

机构地区：[1]河南师范大学化学与环境科学学院,新乡453007

出　　处：《分析试验室》2012年第8期93-95,共3页Chinese Journal of Analysis Laboratory

摘　　要：在酸性条件下,铬黑T、钼酸铵与蛋白质形成聚合物,使体系的共振光散射明显增强。据此建立了利用共振光散射技术测定总蛋白含量的新方法。在最佳条件下,体系的最大散射峰位于555nm处。共振光散射增强的程度与蛋白质的浓度呈良好的线性关系。牛血清白蛋白和人血清白蛋白的线性范围分别为0.20～10.0μg/mL和0.10～8.0μg/mL,检出限为0.050μg/mL和0.039μg/mL。方法已用于人血清样品的分析,并与考马斯亮蓝的测定结果进行了比较,两者无显著性差异。Serum albumin,eriochrome black T and ammonium molybdate form an aggregate in acidic media,which results in the significant enhancement of resonance light scattering（RLS） intensity.A novel method for the determination of protein was developed.Under optimal conditions,the RLS intensity reaches maximum at 555 nm and the enhanced intensities of RLS are proportional to the concentrations of proteins in the ranges of 0.20～10.0 μg/mL and 0.10～8.0 μg/mL for bovine serum albumin（BSA） and human serum albumin（HSA）,respectively.The limits of detection are 0.050μg/mL for BSA and 0.039μg/mL for HSA.The method has been applied to assay human serum samples satisfactorily.The results were compared to those of Coomassie brilliant blue method,and no significant difference was obtained.

关 键 词：蛋白质 钼酸铵 铬黑T 共振光散射 

分 类 号：O657.3[理学—分析化学]