La^(3+)离子对叶绿体CF_1-ATPase活力影响研究  

Influence of La^(3+) Ions on Activity of Chloroplast CF_1-ATPase

作　　者：潘登奎[1] 潘仲光[1] 原向阳[1] 郭平毅[1] 

机构地区：[1]山西农业大学化学生态研究所,山西太谷030801

出　　处：《中国稀土学报》2012年第4期482-487,共6页Journal of the Chinese Society of Rare Earths

基　　金：山西省自然基金项目(093022)资助

摘　　要：为进一步弄清La3+离子对光合磷酸化及Hill反应影响作用机制,采用分光光度法,控制反应液中La3+离子浓度在0～20μmol.L-1范围,对纯化的游离态CF1-ATPase水解ATP活性测定结果表明:La3+离子对CF1-ATPase具有明显的促进作用。而且随La3+离子浓度增大,CF1-ATPase活性动力学曲线明显呈现"S"型。但当La3+离子浓度大于25μmol.L-1之后,La3+离子对游离态CF1-ATPase则表现出一定的抑制作用。而La3+离子浓度在0～30μmol.L-1范围内,受La3+离子作用类囊体膜CF1-ATPase活性动力学曲线则呈现双"S"形曲线。热稳定性实验结果表明:La3+离子可以降低CF1-ATPase活化能,提高CF1-ATPase的热稳定性。La3+离子对CF1-ATPase活性的影响可能与CF1-ATPase分子上别构位点有关,这也可能是La3+离子促进叶绿体Hill反应的关键部位。The results showed that 0-20 μmol · L^-1 concentration range of La^3＋ ion on the heat-DTT activated soluble CF1-ATPase activity in pre-columri prior had obviouly stimulative effect.And with La^3＋ ion concentration increased,CF1-ATPase kinetic curve obviouly appears ＂S＂ model.But when La^3＋ ion concentration in more than 25 μmol · L-1,La^3＋ to the heat-DTT activation soluble ATPase showed certain inhibition.And La^3＋ concentration in 0~30 μmol · L-1 range,La^3＋ ion thick on the heat-DTT activation of thylakoid membrane bound CF1-ATPase activity kinetic curve revealed a double ＂S＂ shape curve.The scope of the activity test results showed that the La^3＋ ion could reduce CF1-ATPase activation energy,and improve CF1-ATPase thermal stability.La^3＋ for heat-DTT activation of the soluble CF1-ATPase,as well as on thylakoid membrane bound CF1-ATPase active effects might CF1-ATPase molecular allosteric site on relevant.And La^3＋ ion might be the key point to promote Hill reaction of chloroplast.But also may be La^3＋ ion promote chloroplast of the key parts of the Hill reaction.

关 键 词：La3+ CF1-ATPase 活性动力学 活化能 

分 类 号：O614.33[理学—无机化学]