6种中药生物碱对内毒素诱导内皮细胞分泌白介素-1α、NO和6-酮-前列腺素F_(1α)的影响  

The Inhibitory Effects of Six Alkaloids for IL-1α,NO and 6-Keto-PGF_(1α) in LPS-Induced Endothelial Cells

作　　者：胡屹屹[1] 何孔旺[1] 温立斌[1] 

机构地区：[1]江苏省农业科学院兽医研究所,江苏南京210014

出　　处：《华南农业大学学报》2012年第3期407-410,共4页Journal of South China Agricultural University

基　　金：江苏省农业科技自主创新基金(0250411025)

摘　　要：通过研究青藤碱、防己诺林碱、水苏碱、川芎嗪、苦参碱和吴茱萸碱6种中药生物碱对细菌内毒素(LPS)作用下猪血管内皮细胞分泌细胞因子白介素-1α(IL-1α)、NO和6-酮-前列腺素F1α(6-keto-PGF1α)的影响,探讨生物碱抗LPS的药理作用机制.培养猪血管内皮细胞至单层融合状态,加入LPS刺激,3 h后加入高、中、低3种质量浓度的上述6种中药生物碱,继续培养21 h后收集细胞上清液,用ELISA测定IL-1α、NO和6-keto-PGF1α的含量.结果显示青藤碱、苦参碱和吴茱萸碱能显著降低内皮细胞分泌IL-1α的水平;青藤碱、水苏碱和川芎嗪能显著降低内皮细胞分泌NO的水平;防己诺林碱能显著降低内皮细胞分泌6-keto-PGF1α的水平.结果提示6种中药生物碱具有明显的抗细菌内毒素作用,可在由IL-1α、NO和6-keto-PGF1α介导的机体炎症、水肿和凝血等病理损伤过程中发挥治疗作用.The aim of the research is to investigate the antiendotoxin effects of sinomenine, fangchinoline, stachydrine, chuanxionggzine, oxymartrine and evodiamine. Endothelial cells were challenged with 1μg/mL LPS for 3 h and treated respectively with six alkaloids at three concentrations （ 1,5 and 10 μg/mL）. The ceils were incubated at 37 ℃ in a cell incubator for 21 h. The supernatants were collected and analyzed for the levels of IL-1α, NO and 6-keto-PGF1α by ELISA kits. The results revealed that sinomenine, oxymartrine and evodiamin inhibited the production of IL-1α; sinomenine, stachydrine and chuanxionggzine inhibited the secretion of NO; fangchinoline down-regulated 6-keto-PGF1α expression. All these changes were significant. Altogether, these data suggested that six alkaloids would exert beneficial therapeutic effects on inflammation, edema and coagulation via these cytokines.

关 键 词：生物碱 内毒素 白介素-1Α 一氧化氮 6-酮-前列腺素F1Α 

分 类 号：S853.74[农业科学—临床兽医学]