蛋白核小球藻Chlorella pyrenoidosa-15的异养培养条件优化及污水养殖  

Optimization of Chlorella pyrenoidosa-15 Photoheterotrophic Culture and Its Use in Wastewater Treatment

作　　者：王秀锦[1] 李兆胜[1] 邢冠岚[1] 李卓凝[1] 袁红莉[1] 杨金水[1] 

机构地区：[1]中国农业大学生物学院,农业生物技术国家重点实验室,北京100193

出　　处：《环境科学》2012年第8期2735-2740,共6页Environmental Science

基　　金：中央高校基本科研业务费专项(2011JS109;2011-BKS-22)

摘　　要：为了提高微藻的生物量及油脂产量以降低微藻生物柴油的生产成本,采用光异养培养模式对蛋白核小球藻进行培养,确定其最适生长的碳源为葡萄糖,氮源为大豆蛋白胨.采用响应面设计的方法对蛋白核小球藻Chlorella pyrenoidosa-15光异养培养过程中的最佳碳氮源浓度进行了优化,在葡萄糖含量为17.53 g.L-1,大豆蛋白胨含量为8.67 g.L-1时,生物量最大产量为0.63 g.(L.d)-1,与模型预测结果[0.62 g.(L.d)-1]基本吻合,此时其油脂含量为19.25%,油脂产量达到121.3mg.(L.d)-1.污水养殖结果显示,在以北京市城市生活污水为培养基的情况下,微藻Chlorella pyrenoidosa-15对污水具有良好的净化能力,COD的去除率达到80.9%,总氮的去除率达到69%,同时其也具有较好的产油效率,生物量和油脂含量分别可达到1.00 g.L-1和24.12%,具有进一步研究的理论及应用价值.To improve the biomass and lipid productivity of the microalgae Chlorella pyrenoidosa-15,the carbon and nitrogen sources were screened to culture it heterotrophically.The best carbon and nitrogen sources were glucose and soy peptone,respectively.The carbon and nitrogen concentrations were optimized with the help of response surface design.The maximum biomass productivity was predicted to be 0.62 g·（L·d）-1 with glucose and soy peptone concentrations of 17.53 g·L-1 and 8.67 g·L-1,respectively.The results of response surface design were validated with biomass productivity of 0.63 g·（L·d）-1 and lipid content of 19.25%.The lipid productivity reached 121.3 mg·（L·d）-1.In the research of Chlorella pyrenoidosa-15 cultured in non-autoclaved Beijing urban wastewater,the maximum algae biomass dry weight of 1.00 g·L-1 was achieved with a lipid content of 24.12%.Results also showed that the treatment using Chlorella pyrenoidosa-15 effectively reduced the COD values and total nitrogen content in the wastewater,with a COD degradation rate of 80.9%,and a 69% decrease in total nitrogen content.

关 键 词：微藻 蛋白核小球藻 异养培养 生物柴油 污水养殖 

分 类 号：X382[环境科学与工程—环境工程]