不同花生品种胚小叶芽诱导的研究  

Study on Shoot Induction from Leaflets of Different Peanut Cultivars(Arachis hypogaea L.)

作　　者：任艳[1,2] 李磊[3] 崔潇[3] 石延茂[2] 王辉[2] 李双铃[2] 袁美[2] 

机构地区：[1]中国海洋大学,山东青岛266100 [2]山东省花生研究所,山东青岛266100 [3]青岛市农业科学研究院,山东青岛266100

出　　处：《山东农业科学》2012年第7期13-15,共3页Shandong Agricultural Sciences

基　　金：科技部"863"计划项目(2006AA100106)

摘　　要：比较了4个花生品种的胚小叶在不同浓度NAA和TDZ诱导培养基中的不定芽诱导率;并观察了用0.1%升汞和10%次氯酸钠不同处理的灭菌效果及其对种子萌发的影响。结果表明:花生种子用75%酒精处理0.5～1 min后用10%次氯酸钠处理15 min灭菌效果理想,萌发率高;诱导培养基中以添加1 mg/LNAA和0.02 mg/L TDZ最有利于不定芽诱导,4个花生品种诱导率都较高,达到80%以上,其中花育20的芽诱导率最高,为89.2%。The shoot induction rate of lobules embryos from 4 peanut cuhivars were media added different concentrations of NAA and TDZ. The and their influences to seed germination were also observed. sterilizing effect of 0.1% HgCI2 The results indicated that the ste compared in the and 10% NaC10 rilizing effect was ideal and the germination rate was higher when the peanut seeds were treated for 0.5 ～1.0 minutes with alcohol and 15 minutes with 10% NaC10. The media added 1 mg/L NAA and 0.02 mg/LTDZ were the most favorable to in vitro shoot induction with the induction rate over 80%. In which, the shoot induction rate of Huayu 20 was the highest as 89.2%.

关 键 词：花生品种 组织培养 种子萌发 芽诱导 

分 类 号：S565.204.3[农业科学—作物学]