一种适合杨树树皮的蛋白质提取方法  

A Protein Extraction Method Suitable for Poplar Bark

作　　者：赵相涛[1] 吕全[2] 赵嘉平[3] 马跃[1] 陈亮[1] 刘会香[1] 张星耀[2] 

机构地区：[1]山东农业大学植物保护学院/山东省林业有害生物防控工程技术研究中心/山东省农业微生物重点实验室,山东泰安271018 [2]国家林业局森林保护学重点实验室/中国林业科学院森林生态环境与保护研究所,北京100091 [3]北京林业大学林学院,北京100083

出　　处：《山东农业科学》2012年第7期121-123,共3页Shandong Agricultural Sciences

基　　金：国家科技部基础性工作专项(2009FY210100)的部分研究内容

摘　　要：双向电泳技术是进行蛋白质组分分离和功能鉴定的重要步骤。提取和制备高质量的蛋白质是进行双向电泳的首要前提。杨树树皮组织中含有大量的干扰物质,影响了蛋白质的提取和双向电泳的分离效果。为建立一种适合杨树树皮蛋白质的双向电泳程序,本实验以107杨为材料,采用改良的三氯乙酸-丙酮沉淀法提取杨树树皮蛋白质,经双向电泳和考马斯亮蓝染色检测,得到了理想的双向电泳图谱,为杨树与病原互作的蛋白质组学研究奠定了基础。Two - dimensional electrophoresis （2 - DE） is an important step for protein separation and function identification. Extracting and preparing high -quality protein is the first premise of 2 -DE. There is a large amount of interfering substances in poplar bark tissues, which influence the extraction of protein and separation effect of 2 - DE. In order to develop a 2 - DE procedure suitable for poplar bark protein, the poplar 107 was used as material. The improved method of trichloroacetic acid （TCA） -acetone was adopted to extract the proteins of poplar bark. After the two - dimensional electrophoresis and detection with Coomassie brilliant blue, the desirable gel maps were obtained. It layed the foundation for proteomics research of poplar and pathogen interaction.

关 键 词：蛋白质组学 杨树树皮 双向电泳技术 

分 类 号：O658.9[理学—分析化学]