HBeAg阴性乙型肝炎患者HBV-LP及HBV-DNA检测分析  

Detection of serum HBV-LP and HBV-DNA in hepatitis B patients of negative HBeAg

作　　者：刘鸿生[1] 胡志刚[1] 俞蕾[1] 周颖[1] 

机构地区：[1]南京医科大学附属无锡人民医院医学检验科,江苏南京214023

出　　处：《中华医院感染学杂志》2012年第13期2743-2745,共3页Chinese Journal of Nosocomiology

摘　　要：目的分析HBeAg阴性的乙型肝炎患者血清HBV-LP及HBV-DNA检测的价值。方法收集64份HBeAg阴性的乙型肝炎患者血清标本,分别采用实时荧光定量PCR检测HBV-DNA、ELISA法检测HBV-LP。结果 64例HBeAg阴性的乙型肝炎患者血清中,HBV-DNA阳性36例,总检出率为56.3%;HBV-LP阳性44例,总检出率为68.8%;HBV-DNA与PreSl抗原具有较好的相关性,HBeAg阴性的乙型肝炎患者血清HBV-DNA、HBV-LP阳性率差异无统计学意义,HBV-DNA复制拷贝数为<103、103-5、106-7、>107拷贝/ml,HBV-LP检出率分别为60.7%、82.6%、60.0%、66.7%。结论 HBeAg阴性的乙型肝炎患者血清中HBV-LP检测可以作为HBV-DNA检测的有效补充。OBJECTIVE To analyze the clinical significance of detection of serum HBV-LP and HBV-DNA in patients with hepatitis B whose HBeAg were negative.METHODS A total of 64 serum specimens of hepatitis B patients with negative HBeAg were collected.HBV-DNA was detected using the real-time fluorescent quantitative PCR and HBV-LP was measured by ELISA.RESULTS Of the 64 serum specimens,36 were positive for HBV-DNA and 44 were positive for HBV-LP with the detection rates of 56.3% and 68.8%,respectively.It showed that HBV-LP and HBV-DNA were related but there was no significant difference between their own positivity.The HBV-DNA copies being 107 copy/ml,the detection rates of HBV-LP were 60.7%,82.6%,60.0% and 66.7%,respectively.CONCLUSION Detection of HBV-LP can serve as an effective complement to the measurement of HBV-DNA in patients with Hepatitis B whose HBeAg were negative.

关 键 词：乙型肝炎 乙型肝炎病毒大蛋白 乙型肝炎病毒DNA 

分 类 号：R512.62[医药卫生—内科学]