PEG-6000模拟干旱及NaCl和温度胁迫对不结球白菜种子活力的影响  

Effect of Vigor Index of Non-heading Chinese Cabbage Seeds under PEG-6000 Simulating Drought,NaCl and Temperature Stress

作　　者：吴汉花[1,2] 曹雪[1,2] 蒋芳玲[1,2] 吴震[1,2] 

机构地区：[1]农业部南方蔬菜遗传改良重点开放实验室,江苏南京210095 [2]南京农业大学园艺学院,江苏南京210095

出　　处：《种子》2012年第7期1-6,12,共7页Seed

基　　金：国家科技支撑计划项目(编号:2008 BADB 1 B06)

摘　　要：以不结球白菜品种矮脚黄种子为材料,研究聚乙二醇(PEG-6000)模拟干旱胁迫、NaCl胁迫和温度胁迫下,种子萌发和内部抗氧化相关特性及其渗透调节物质的变化。结果表明:不结球白菜种子的发芽势、发芽率、发芽指数和活力指数随3种胁迫的加重而显著下降,不正常苗率显著上升;随着3种胁迫的加重,SOD和POD活性均下降,CAT活性先有上升的趋势,胁迫加重时降低,活性氧产生速率加快,H_2O_2积累,造成生物膜破坏,相对电导率上升;逆境胁迫下,渗透调节物质可溶性糖、可溶性蛋白和脯氨酸含量显著增加。综上说明,不结球白菜种子在PEG-6000模拟干旱胁迫、NaCl胁迫和温度胁迫下,其种子抗氧化系统代谢失去协调作用的能力,并造成活性氧积累伤害,渗透调节紊乱,从而降低了种子活力直至种子死亡。In this study, using non-heading Chinese cabbage （ Brassica campestris ssp. chinensis Makino. ） variety Aijiaohuang as material, seed germination, antioxidant-related indexes and osmoregulation substance were studied undre PEG-6000 simulating drought, NaCl and temperature stress. The results showed that： germination energy, germination rate, germination index and vigor three stresses, but abnormal seedling increased. With increasement index decreased with increasement of the of the three stesses, superoxide dismutase （SOD） and peroxidase （POD） activity decreased, catalase （CAT）activity firstly went up and then went down, active oxygen production speed increased, the hydrogen peroxide （H202） accumulated, so biofilm was damaged and the relative electrolyte leakage was increased. In the adversity stress, soluble suger, soluble protein and proline content increased. Comprehensive analysis showed that under the PEG-6000 simulating drought, NaCl and temperature stress, the disorder of antioxidant system led to reactive oxygen metabolism imbalance, the ability of osmotic adjustment was disorder, so caused by seed vigor decreased and even death.

关 键 词：不结球白菜 PEG-6000模拟干旱 NACL胁迫 温度胁迫 种子活力 

分 类 号：S634.3[农业科学—蔬菜学]