Frizzed家族基因mRNA在小鼠动情周期卵巢组织中的表达规律  

Relative mRNA Expression Levels of Frizzed Family Genes in Mice Ovaries at Different Estrous Stages

作　　者：王少兵[1,2] 徐银学[2] 王正朝[2,3,4] 

机构地区：[1]芜湖职业技术学院,安徽芜湖241000 [2]南京农业大学动物科技学院,江苏南京210095 [3]福建师范大学生命科学学院,福建福州350108 [4]福建省发育与神经生物学重点实验室,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2012年第4期76-81,共6页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家863高技术研究发展计划资助项目(2006AA10Z136);国家自然科学基金资助项目(31101032);教育部博士点基金资助项目(20113503120002);福建省自然科学基金资助项目(2011J01144);福建省教育厅杰出青年基金资助项目(AJ11041);芜湖职业技术学院引进人才科研基金资助项目

摘　　要：运用Real-time Q-PCR方法分析了Frizzed1-10mRNA在小鼠动情周期卵巢中的相对表达水平.结果发现10个Frizzed家族基因中,除Frizzed8外,其他均有不同水平的表达.其中Frizzed1和2在动情前期表达水平显著高于其他3个时期水平,约是间情期水平的4倍(P<0.05);动情前期和间情期的Frizzed3表达水平约是另2个时期的1.5倍(P<0.05);Frizzed4在动情期表达水平显著高于其他3个时期,约是间情期的3倍(P<0.05);间情期Frizzed5表达水平显著高于动情前期和动情期,约是动情期的5倍(P<0.05);Frizzed7表达类似于Frizzed5,间情期表达水平约是动情前期的4倍(P<0.05);Frizzed10的表达在动情前期表达水平约是动情期的3倍(P<0.05);Frizzed6和9在4个时期的表达无显著差别(P<0.05).以上结果表明:Frizzeds在4个动情时期卵巢中广泛而差异表达,并推测Frizzed1—4可能在动情周期卵泡发育过程中具有重要作用.Real-time Q-PCR was used to analyze the relative expression levels of Frizzed 1-10 mRNAs in the mice ovaries at different estrous stages.The results showed that they had different expression patterns except Frizzed 8.Expressions of Frizzed 1 and 2 were the highest in proestrus and both were about 4 times higher than those in diestrus（P〈0.05）.Frizzed 3 was 1.5 fold higher in proestrus and diestrus than those in the other two periods（P〈0.05）.Frizzed 4 was found significantly higher expression in estrus,which is about 3 fold than that in diestrus（P〈0.05）.Frizzed 5 was marked higher in diestrus than those in proestrus and estrus,being approximately 5 times higher than that in estrus（P〈0.05）.Frizzed 7 was similar to Frizzed 5,about 4 fold higher than the level in proestrus（P〈0.05）.Frizzed 10 expression in proestrus was about more than 3 times higher than that in estrus（P〈0.05）,but Frizzed 6 and 9 were no significant difference during the estrous cycle（P〉0.05）.These results suggested that Frizzeds were widely and differentially expressed in estrous ovary and Frizzed 1—4 may play a more important role in the development of follicle during the estrous cycle.

关 键 词：Frizzed 动情周期 卵巢 小鼠 

分 类 号：Q492.5[生物学—生理学]