大鼠挫伤型急性脊髓损伤模型的制备及死亡原因分析  

Establishment of rat model of acute spinal cord injury and analysis of death causes

作　　者：黄怀[1] 张旭[1] 陈辉强[2] 古菁[1] 叶水林[1] 廖晃怡[3] 

机构地区：[1]广州军区广州总医院高压氧科,广东广州510010 [2]广州军区广州总医院骨科医院,广东广州510010 [3]南方医科大学附属新会医院高压氧治疗中心,广东江门529100

出　　处：《海南医学》2012年第15期23-25,共3页Hainan Medical Journal

基　　金：广东省自然科学基金资助项目(编号:9151001002000005);广东省2010年科技计划项目(编号:2010B031600264)

摘　　要：目的总结脊髓损伤(Spinal cord injury,SCI)标本成功制备需要特别注意的事项,为SCI的实验研究提供有价值的参考。方法 60只SD大鼠随机分为加强饲养组(A组)和常规饲养组(B组),两组均采用改良Allen法打击造成急性挫伤型SCI模型,分别实施预定饲养措施饲养1周,观察其并发症及死亡情况。结果模型制备后第1天A组和B组大鼠的BBB评分、斜坡试验角度值差异均无统计学意义(P>0.05),模型制备后第7天,A组和B组大鼠的BBB评分、斜坡试验角度值差异有统计学意义(P<0.05)。A组大鼠死亡率为26.67%,B组死亡率为56.67%,B组大鼠死亡率明显高于A组。结论严格、恰当的制备措施能够明显降低挫伤型SCI大鼠模型的死亡率,有效提高样本量。Objective To analyze and summarize the best way to establish the rat model of acute spinal cord injury （SCI）, and to provide reference for the study of SCI. Methods Sixty Sprague-Dawley rats were randomly di- vided into group A and group B, with 30 rats in each group. Group A adopted positive nursing measures, while group B applied routine nursing. The mortality and complications were observed after one week. Results The BBB scores and slope angle values showed no statistically significant difference between the two groups on the first day after the establishment of the rat model （P〉0.05）, while showed statistically significant difference on the seventh day after the establishment （P〈0.05）. The mortality of group A was 26.67%, significantly lower than 56.67% of group B （P〈0.05）. Conclusion Effective nursing measures can raise the survival rate and reduce the mortality, thus is helpful to the animal experiment.

关 键 词：SCI 大鼠模型 饲养 

分 类 号：R332[医药卫生—人体生理学]