牛耳枫药材中总黄酮含量测定  

Determination of total flavonoids in Daphniphylium calycinum

作　　者：李海龙[1] 刘明生[1] 张俊清[1] 符乃光[1] 陈峰[1] 

机构地区：[1]海南医学院药学院 海南省热带药用植物研究开发重点实验室,海南海口571101

出　　处：《海南医学》2012年第15期106-108,共3页Hainan Medical Journal

摘　　要：目的建立测定牛耳枫药材中总黄酮的含量方法。方法以芦丁为对照，显色后采用分光光度法于510nm处测定总黄酮含量。结果总黄酮在7．84-47．04μg／ml（r=0．9998）范围内线性关系良好；总黄酮的平均回收率为98．51％，RSD=2．48％。结论建立的方法可用于牛耳枫总黄酮的测定。Objective To establish the methods for the determination of total flavonoids in Daphniphylium ealycinum. Methods The total flavonoids was determined by visable spectrophotometry with the detection wave- length of 510 nm after color development reaction, using turin as the control. Results The standard curve was linear in the range of 7.84-47.04 μg/mL for total flavonoids （r=0.999 8）, and the average recovery of total flavonoids was 98.51% with RSD of 2.48%. Conclusion The method established can be used to determine the content of total flavo- noids in Daphniphylium calycinttm and to control the quality of Daphniphylium calycinum.

关 键 词：牛耳枫 分光光度法 总黄酮 含量 测定 

分 类 号：R28[医药卫生—中药学]