加工型辣椒花药培养技术研究  

Study on the Anther Culture of Processing Pepper (Capsicum annuum L.)

作　　者：静一[1] 黄启中[2] 罗安才[3] 雷开荣[1] 黄任中[2] 吕中华[2] 郑勇[2] 林清[1] 

机构地区：[1]重庆市农业科学院生物技术研究中心/逆境农业研究重庆市市级重点实验室,重庆401329 [2]重庆市农业科学院蔬菜花卉研究所,重庆400055 [3]重庆师范大学生命科学院,重庆400047

出　　处：《中国农学通报》2012年第22期144-150,共7页Chinese Agricultural Science Bulletin

基　　金：重庆市科委良种创新工程"优势蔬菜高效育种新技术研究与示范"(CSTC;2010AA1023);公益性行业(农业)科研专项"干制辣椒品种优化及安全高效生产关键技术研究与示范"(200903025)

摘　　要：为了满足重庆辣椒高效育种与良种创新的需要,以2个加工型辣椒材料578和186及一个甜椒品种‘海丰28’为试材进行花药培养研究。研究了预处理与预培养的时间与温度,并通过正交试验设计,探讨了基本培养基、植物生长调节物质、活性炭、硝酸银、碳源等主要因子对辣椒花药培养的影响。结果表明,高温预培养35℃7天时辣椒578、186、甜椒‘海丰28’的植株诱导率分别为4.86%、2.50%、0.56%,是对3种材料都适合的预培养条件。通过正交试验分析得到对2个基因型特异的优化培养基配方,并且通过比较得到一个对2个基因型都较适合的培养基配方。对花药培养再生的植株进行倍性鉴定,证明3个基因型均得到了具有12条染色体的单倍体植株。以饱和对二氯苯与0.2%秋水仙素浸泡生长点24h进行加倍,成功获得了双单倍体植株。In order to meet the needs of the Chongqing pepper efficient breeding and germplasm innovation,pepper anther culture research was studied.Two processing pepper varieties 578 and 186 and a sweet pepper variety ’ Haifeng 28 ’ were selected as the experimental materials.The time and temperature of the pretreatment and pre-incubation were studied.By the orthogonal experimental design we explored the effects of the basic medium,plant growth regulating substances,activated carbon,silver nitrate,carbon source on anther culture.The results indicated that 35℃ for 7 days was the suitable pretreatment condition for all the three materials,the plant induction rates of pepper 578,186,sweet pepper ’ Haifeng 28 ’ were 4.86%,2.50%,0.56%.Two genotype-specific optimization of culture medium were established by orthogonal test analysis and one culture medium suited to the two genotypes was obtained.The ploidy identification showed that haploid plantlets with 12 chromosomes were regenerated from anthers of all the three genotypes.Socking the growing point with the mixture of 0.2% colchicine and saturated p-dichlorobenezene for 24 hours,double haploid plants were successfully obtained.

关 键 词：辣椒 花药培养 胚状体诱导 愈伤组织诱导 

分 类 号：S-3[农业科学]