不同种类和浓度抗冻保护剂对虾夷马粪海胆精子的影响  

Effect of Different Types and Concentrations of Cryoprotectants on Strongylocentrotus intermediusone Sperm

作　　者：商国良[1] 丛晓霏[1] 丁君[1] 常亚青[1] 

机构地区：[1]大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023

出　　处：《现代农业科技》2012年第15期252-255,共4页Modern Agricultural Science and Technology

基　　金：国家自然科学基金(30972269);辽宁省科技计划(2007203004);辽宁省"百千万人才工程"资助项目(2008921059);辽宁省渔业厅项目(200916)

摘　　要：以自然海水为基础溶液,用二甲亚砜、乙二醇、甘油分别配制不同体积分数的抗冻保护剂溶液,用以激活虾夷马粪海胆精子并且观察其精子活力、受精率及其形态学上的改变。结果表明,经4%~18%的抗冻保护剂处理过的样本精子皆可被激活,并具有受精能力,若抗冻保护剂浓度达到18%以上,则精子全部失活。经4%乙二醇处理过的样本精子活力为最高,平均可达到76.44%（P≤0.05）,但在各浓度梯度的样本中均以二甲亚砜处理过的样本受精率最高。这3种抗冻保护剂均对虾夷马粪海胆精子有毒害作用,并且对其精子的活力和受精率有一定的影响。Using natural sea water as the basic menstruum,the cryoprotectant solutions were prepared by dimethyl-sulfoxide, ethylene glycol and glycerol with different volume fractions respectively. The Strongylocentrtus intermedius sperm was activated by utilizing the solution and observe its motility, fertilization rate as well as its morphological changes. The experimental results demonstrated that all of sperm treated by cryoprotectant solution of concentration level 4%-18% could be activated and possessed the fertilization ability as well. The cryoproteetant samples with concentration level above 18% had toxicity which could deactivate all the sperm ,while the sperm motility of the samples treated by 4% ethylene glycol was the highest, which could reach the average level of 76.44% （P ≤ 0.05 ）. However, the fertilization rate of the samples treated by dimethyl-sulfoxide was the highest in each concentration level. These three cryoprotectants were all poisonous to Strongylocentrotus intermedius sperm,and the sperm motility and fertilization rate both declined after being treated.

关 键 词：虾夷马粪海胆 抗冻保护剂 精子活力 受精率 

分 类 号：Q959.26[生物学—动物学]