兔胆红素钙结石成石过程中血清、胆汁免疫球蛋白和胆囊免疫球蛋白分泌细胞含量的变化  

A Study of the Immune in Formation of Calcium Bilirubinate Gallstones in Different Rabbit Models-The Changes of the Immunoglobulins in Serum and Bile and the Immunoglobulins Forming Cells in the Gallbladder Mucoderm

作　　者：曾勇[1] 肖路加[1] 姚辉华[1] 何嘉兵[2] 

机构地区：[1]华西医科大学附属第一医院肝胆外科,成都610041 [2]四川省广元市人民医院外科

出　　处：《华西医科大学学报》2000年第2期155-158,共4页Journal of West China University of Medical Sciences

基　　金：高等学校博士学科点专项基金

摘　　要：为动态观察胆红素钙结石成石过程中免疫球蛋白的变化，探讨在成石过程中免疫球蛋白的作用，将100只日本大耳白兔随机分为正常对照组（Con组，n＝10），胆道不全梗阻组（BO组，n＝45）和胆道不全梗阻加感染组（BOI组，n＝45），动态检测实验组术后3、7、14和20天的胆汁，血清免疫球蛋白（Ig）及胆囊Ig分泌细胞的含量，并比较其变化。结果：实验组各时相血清IgA浓度均明显高于Con组（P＜0．001），血清中IgG浓度随结石形成增加而逐渐升高。胆汁细菌培养阴性者胆汁中IgA和IgG浓度高于Con组（P＜0．05），胆汁细菌培养阳性者胆汁中Ig浓度略低于Con组。Con组免胆囊粘膜存在少量的Ig分泌细胞，BO组胆囊粘膜IgG和IgA分泌细胞无明显变化，BOI组Ig分泌细胞明显高于Con组（P＜0．001），以InG分泌细胞最为明显。以上结果提示：兔胆红素钙结石成石过程中，血清和胆汁Ig有明显改变，而胆汁中Ig的含量与细菌感染密切相关，IgA在成石过程中起着主要作用。The changes of Ig-forming cells in the gallbladder mucoderm and the imunoglobulins in serum and bile were studied in the rabbit models. One hundred rabbits were randomly divided into the control group (Con, n = 10), simple biliary obstruction group (BO, n = 45) and biliary obstruction and infection group (BOI, n = 45).The results showed that the concentrations of serum immunoglobulin A (IgA) in BO and BOI groups increased remarkably in all phases (P<0. 001), The councentratcons of serum IgG in both groups increased with the formation of gallstones. The IgA and IgG contents of bile samples in BO and BOI groups with negative bacterial culture were much higher than those of control group (P<0.05), but the Ig content of bile with positive culture was slightly lower than that of the control group. Only a few Ig-forming ceIls were found in the gallbladder mucoderm of normal rabbits. The counts of Ig-forming cells in the mucoderm in BO group remained unchanged,but increased much higher in BOI group (P<0. 001), especially in IgG formation. This expereiment demonstrates the immunoglobulins of serum and bile change significantly during the formation of gallstones. The Ig content of bile has a relationship with bacterial infection. IgA plays an important role in gallstone formation. The gallbladder of rabbit may be an important place of Ig-formation. The quantity of Ig forming cells in biliary tract may have a close relationship with the gallstone formation.

关 键 词：兔 胆红素钙结石 免疫球蛋白 分泌细胞 

分 类 号：R392.12[医药卫生—免疫学]