建鲤IGFBP1基因的克隆及与增重相关的SNP位点分析  

Isolation of IGFBP1 and SNP Loci Related to Body Weight Gain in Cyprinus carpio var.jian

作　　者：魏可鹏[1] 俞菊华[1,2] 李红霞[2] 李建林[2] 唐永凯[2] 夏正龙[1] 

机构地区：[1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心,农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,江苏无锡214081

出　　处：《华北农学报》2012年第3期75-80,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家支撑计划项目(2006BAD13B09);农业部公益性行业专项(200903045);现代农业产业技术体系建设专项(nycytx-49)

摘　　要：旨为查找建鲤(Cyprinus carpio var.jian)胰岛素样生长因子结合蛋白1(Insulin-like growth factor binding pro-teins 1,IGFBP1)基因上与增重相关的SNP位点。利用基因克隆,Clustal W比较8个个体的DNA序列,筛选SNP位点。并采用PCR-RFLP方法检测了其中2个位点(1b-E1-A210G,1b-E3-C108T)在913尾建鲤(♂469,♀444)中的基因型分布。成功分离得到了建鲤IGFBP1的2条DNA序列,并在1a、1b上分别找到7个和24个SNPs位点,其中,1a外显子上有2个,1b外显子上有4个。检测的2个位点与增重的相关性分析表明:2位点均与雄鱼增重呈极显著相关(P<0.01),其中,增重快的基因型在鱼种阶段分别为GG型和CC型,在成鱼阶段分别为AG型和CC型。与雌鱼鱼种阶段增重相关的位点为A210G,其AG型增重极显著快于AA型(P<0.01),与雌鱼成鱼阶段增重相关的位点为C108T,其CC型、CT型增重极显著快于TT型(P<0.01)。对组合成的9种双倍型与增重的相关性分析表明:雌雄鱼中双倍型D4、D5、D7个体生长显著(P<0.05)或极显著(P<0.01)快于双倍型D6、D9个体,D4、D5、D7双倍型个体在整个样品中占60%,还存在改良空间。试验检测的2个位点A210G、C108T可作为建鲤增重相关的分子标记应用于育种实践。Two IGFBP1 genes were isolated from Cyprinus carpio var.jian.Seven and twenty-four SNP loci were identified on IGFBP1a and IGFBP1b,respectively.There are two and four SNPs were located at exons of 1a and 1b,respectively.PCR-RFLP was applied to detect genotypes of 913 individuals（♂469,♀444） at two SNPs（1b exon 1_A210G,1b exon 3_C108T）.Correlation analysis between genotypes and weight gain showed：both two SNPs were associated with male weight gain significantly（P〈0.01）.To these two SNPs loci,GG and CC genotype individuals grew faster than other individuals in the juvenile stage and it was AG and CC genotype in the adult stage.A210G was also significantly related to female juvenile weight gain（P〈0.01） and AG individuals grew significantly faster than AA ones.The CC and CT type male adult weight gains of C108T were significantly（P〈0.01） larger than TT type.Nine diplotypes were found in this experimental population.Multiple comparison results displayed that the body weight gains of D4,D5,D7 were significantly larger than those of D6,D9.D4,D5 and D7 individuals accounted for sixty percent in experimental population,so there still are spaces for further improved.A210G and C108T identified in this experiment can be used in molecular breeding of Cyprinus carpio var.jian.

关 键 词：建鲤 IGFBP1 SNP 增重 相关性 

分 类 号：Q953[生物学—动物学] Q78