绵羊MSTN基因启动子区SNPs分析  

Analysis of SNPs in Promoter Region of Myostatin in Sheep

作　　者：贺三刚[1] 陈芯[1,2] 刘晨曦[1] 刘国松[1,2] 刘明军[1] 

机构地区：[1]新疆畜牧科学院生物技术研究中心,农业部草食家畜繁育生物技术重点开放实验室,新疆维吾尔自治区动物生物技术重点实验室,新疆乌鲁木齐830000 [2]新疆农业大学农学院,新疆乌鲁木齐830052

出　　处：《华北农学报》2012年第3期81-85,共5页Acta Agriculturae Boreali-Sinica

基　　金：新疆维吾尔自治区国际科技合作项目(200741117)

摘　　要：采用测序和PCR-RFLP的方法,分析了MSTN基因启动子区在7个品种绵羊中的单核苷酸多态性,结果表明:存在2个SNP位点(-959T/C,-784G/A),表现为6种基因型(TT、TC、CC、GG、GA、AA),在-959T/C位点中,国外肉用绵羊品种陶赛特、德国肉用美利奴及我区的巴士拜羊,TT为优势基因型,地方品种绵羊巴音布鲁克羊、多浪羊和阿勒泰羊在该位点中CC为优势基因型,经χ2检验,其群体的基因频率和基因型频率均处于Hardy-Weinberg平衡状态,群体遗传多态性分析表明,这6个绵羊品种中群体的多态信息含量(PIC)均处于0.25～0.50之间,为中度多态,特克赛尔羊在该位点未发生突变。在-784G/A位点中,除陶赛特与特克赛尔羊在该位点未发生突变,其他5个品种绵羊优势等位基因均为G等位基因。经χ2检验后,其群体的基因频率和基因型频率均处于Hardy-Weinberg平衡状态,群体遗传多态性分析表明,多浪羊、巴士拜羊和巴音布鲁克羊的群体多态信息含量(PIC)均处于0.25～0.50之间,为中度多态,而阿勒泰羊和德国肉用美利奴的群体多态信息含量(PIC)小于0.25,为低度多态。Sequencing along with PCR-RFLP was applied to analyze the polymorphism of MSTN gene promoter in seven sheep breeds.Two SNPs at-959（T/C）and-784（G/A） including six genotype（TT,TC,CC,GG,GA,AA）respectively were detected in the promoter region,At-959（T/C）,the predominant genotype of TT was identified in Dorset,German Merino and Bashibai sheep,the CC in Bayinbuluke,Duolang and Aletai sheep.A chi-square analysis suggested that the allele frequencies and genotype frequencies of two polymorphisms were in Hardy-Weinberg equilibrium.Statistical analysis also revealed the variation of intermediate polymorphism information content（PIC） in six breeds（0.25〈PIC〈0.50）.At-784（G/A）,The G was predominant allele in all breeds,whereas the low polymorphism information content（PIC） was observed in German Meilin and Aletai populations（PIC〈0.25） and intermediate PIC in the Bayinbuluke,Duolang and Bashibai populations（0.25〈PIC〈0.50）.

关 键 词：绵羊 MSTN 启动子 PCR-RFLP 多态性 

分 类 号：Q78[生物学—分子生物学]