高效液相色谱法测定朝药材万年蒿中主要有效组分的含量  

Quantification of effective ingredients in the Artemisia sacrorum Ledeb by reverse phase-high performance liquid chromatography

作　　者：郑光浩[1] 徐影 耿聪[3] 姜英子[1] 

机构地区：[1]延边大学药学院,吉林延吉133002 [2]北京星昊医药股份有限公司,北京100176 [3]吉林大学药学院,吉林长春130021

出　　处：《延边大学医学学报》2012年第2期113-116,共4页Journal of Medical Science Yanbian University

摘　　要：[目的]采用高效液相色谱法测定传统朝药材万年蒿中东莨菪内酯、槲皮素及木犀草素的含量.[方法]采用Diamonsil C18色谱柱(250.0mm×4.6mm,5μm),流动相为甲醇-250mL/L磷酸(4∶1)水溶液,流动速度为1.0mL/min,柱温为35℃,检测波长为360nm,线性梯度洗脱.[结果]以峰面积(Y)对进样量(X)进行线性回归,东莨菪内酯、槲皮素和木犀草素的回归方程分别为Y=81 267 X+2 396.7(r=0.999 9),Y=41 356 X-5 037.3(r=0.999 7),Y=13 705 X-741.43(r=0.999 1);线性范围分别为0.106～0.742,0.104～0.728,0.024～0.168μg;加样回收率(n=9)分别为101.38%,RSD为1.94%,101.57%,RSD为1.64%,100.89%,RSD为1.72%.[结论]高效液相色谱法的精密度、稳定性、重复性和加样回收率均较好.所建立的高效液相色谱法具有准确、快速等优点,可用于朝药材万年蒿中3种有效组分的含量测定.OBJECTIVE To determine the contents of three effective ingredients in the Artemisia sacrorum Ledeb by reverse phase-high performance liquid chromatography（RP-HPLC）.METHODS RP-HPLC analysis was performed on that chromatographic column was a Diamonsil-C18（250.0mm×4.6mm,5μm）,the mobile phase was a mixture of methanol-0.4% phosphoric acid aqueous solution in gradient elution,the flow rate was 1.0mL/min,the column tempratrue was 35℃,and the detection wavelength was 360nm.RESULTS The carlibration curves of scopoletin,quercetol,and luteolol were linear in the range of 0.106-0.742,0.104-0.728 and 0.024-0.168μg,and their regressive equations were Y=81267X＋2396.7（r=0.9999）,Y=41356X-5037.3（r=0.9997）,Y=13705X-741.43（r=0.9991） based on their integral area to their concentration,respectively;and their average recovery rate（n=9）was 101.38%（RSD=1.94%）,101.57%（RSD=1.64%）and 100.89%（RSD=1.72%）.CONCLUSION The method is accurate,sensitivite and reliable,and the three effective ingredients from Artemisia sacrorum Ledeb.can be rapidly and accurately quantified by RP-HPLC.

关 键 词：蒿属 色谱法 高压液相 有效组分 

分 类 号：R284.1[医药卫生—中药学]