血管内皮生长因子基因治疗兔下肢缺血的实验研究  

Gene therapy of vascular endothelial growth factor in treatment of hindlimb ischemia in a rabbit model

作　　者：顾洪[1] 陈少萍[2] 张电波 王官将[1] 毛积芳[1] 

机构地区：[1]第二军医大学基础医学部生物化学与分子生物学教研室,上海200433 [2]长海医院心血管内科 [3]长征医院放射科

出　　处：《第二军医大学学报》2000年第5期447-449,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金资助项目!(39780025)

摘　　要：目的 :了解血管内皮生长因子 (VEGF)基因治疗兔下肢缺血模型后新生血管和侧支循环形成状况。 方法 :构建h VEGF1 6 5 的真核表达载体 ,通过直接肌肉注射将 h VEGF1 6 5 基因转染缺血部位肌细胞 ,经颈总动脉插管行下肢血管造影。结果 :VEGF治疗组在质粒转染后 2和 4周动脉血管造影显示 ,新生血管数目和侧枝血管形成较对照组明显增加。 结论 :肌细胞能够吸收裸露的 VEGF c DNA质粒并获得局部高效表达 。Objective:To study the angiogenesis and collateral circulation formation in a rabbit model of hindlimb ischemia after gene therapy of VEGF. Methods:The hVEGF 165 cDNA was cloned into eukaryotic expression vector pcDNA 3. The pcDNA 3/hVEGF 165 was administered intramuscularly, and angiography was performed through the common carotid artery. Results:The angiography was performed 2 and 4 weeks after the initiation of therapy. In VEGF treated animals, neovessels and collateral artery development were more evident compared with controls. Conclusion:Naked DNA of VEGF can be absorbed by muscle cells, and expressed efficiently and locally, to stimulate angiogenesis and collateral circulation in vivo .

关 键 词：侧支循环 血管内皮生长因子 基因治疗 下肢缺血 

分 类 号：R654.405[医药卫生—外科学] R394[医药卫生—临床医学]