三种分子分型技术在嗜肺军团菌分型中的应用与评价  

Application and evaluation of three genotyping methods for Legionella pneumophila

作　　者：张颖[1] 巩向丽[2] 张健[1] 屈平华[3] 庞杏林[1] 陈守义[1] 傅松哲[4] 王鸣[1] 

机构地区：[1]广州市疾病预防控制中心,广东广州510440 [2]中山大学中山医学院 [3]广东省中医院医院 [4]南昌市疾病预防控制中心

出　　处：《中国预防医学杂志》2012年第7期515-519,共5页Chinese Preventive Medicine

基　　金：广州市医药卫生科技项目(201102A213249);广州市科技与信息化局科技攻关项目(2010J-E391)

摘　　要：目的对脉冲场凝胶电泳方法 (PFGE)、基因序列分型(SBT)和mip基因分型方法在嗜肺军团菌分型研究中的分辨力和潜在价值进行比较和论述。方法采用PFGE、SBT和mip基因分型方法对29株嗜肺军团菌和1株标准菌株ATCC33153进行分型比较。结果 PFGE可将30株嗜肺军团菌分为5大类群,19个PFGE型,分辨力为0.9586;SBT可分为22个ST型,分辨力为0.9609;mip基因分型可分为9个型别,分辨力为0.8344。血清型LP1和LP14菌株具有相同的PFGE和mip基因型别,相同或相近的SBT型别。结论 SBT较PFGE具有稍高的分辨力,适用于全球数据库比对和进化亲缘关系的研究,结合PFGE和SBT分型方法有利于流行病学溯源分析。Objective To compare the discriminatory ability and potential value of three widely used methods for the genotyping of Legionella pneumophila.Methods A total of 29 strains of L.pneumophila and one standard ATCC33153 were genotyped simultaneously by pulsed-field gel electrophoresis（PFGE）,sequence-based typing（SBT） and mip sequence.Results There were 5 clusters and 19 PFGE types identified by PFGE with discriminatory index（D） of 0.9586,while 22 sequence types（STs） found by SBT with D value of 0.9608.However,only 9 types were identified by mip gene typing with D value of 0.8344.Several strains with different serotype（LP1 and LP14） identified by SBT,PFGE and mip had very closely related genetic backgrounds,which suggested a possible ＂antigen switching＂ among them.Conclusions For Legionella pneumophila genotyping,SBT is better than PFGE because of the relatively higher discriminating ability which makes it possible to differentiate most of L.pneumophila strains and to be used in the comparison as well as evolutionary relationship study in the global alignment database,while the combination of PFGE and SBT can be used in tracing the epidemiological resource of Legionella.

关 键 词：嗜肺军团菌 脉冲场凝胶电泳 基因序列分型 mip基因分型 

分 类 号：R378[医药卫生—病原生物学]