Determination of catecholamines by ion chromatography coupled to acidic potassium permanganate chemiluminescence detection  

作　　者：Hong Wei Wu Mei Lan Chen Dan Shou Yan Zhu 

机构地区：[1]Department of Chemistry,Xixi Campus,Zhejiang University,Hangzhou 310028,China [2]Department of Chemistry,Xinxiang Medical University,Xinxiang 453003,China [3]College of Biology and Environment Engineering,Zhejiang Shuren University,Hangzhou 310015,China [4]ZheJiang Academy of Traditional Chinese Medicine,Hangzhou 310007,China

出　　处：《Chinese Chemical Letters》2012年第7期839-842,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.20775070);the Natural Science Foundation ofZhejiang Province(Nos.R4080124 and Y5100280);the Analysis and Tesfing Foundation of Zhejiang Province(No.2011C37034)

摘　　要：A simple,fast,sensitive,highly selective and eco-friendly analytical method for the determination of catecholamines in human urine by ion chromatography（IC） with chemiluminescence（CL） detection was described in this paper.Using 12 mmol/L H_2SO_4 without any organic additive as eluent,three catecholamines including epinephrine（EP）,norepinephrine（NE） and dopamine（DA） were well separated on a cation-exchange column.The CL detection was based on the reaction of analytes with acidic potassium permanganate in the presence of formaldehyde as an enhancer.The absence of methanol and acetonitrile in eluent made the proposed method more sensitive and eco-friendly.Under the optimal conditions,the linear range of the proposed method was in the range of 0.02-0.5μg/mL.The limit of detection（LOD） was in the range of 0.6 and 5.1μg/L.The relative standard deviations （RSD） for 0.1μg/mL mixed standard solution were in the range of 0.8-1.9%（n = 11）.The method has been applied to the determination of catecholamines in human urine successfully.Excellent spiked recoveries were achieved for catecholamines ranged from 91.2%to 112.7%.A simple,fast,sensitive,highly selective and eco-friendly analytical method for the determination of catecholamines in human urine by ion chromatography（IC） with chemiluminescence（CL） detection was described in this paper.Using 12 mmol/L H_2SO_4 without any organic additive as eluent,three catecholamines including epinephrine（EP）,norepinephrine（NE） and dopamine（DA） were well separated on a cation-exchange column.The CL detection was based on the reaction of analytes with acidic potassium permanganate in the presence of formaldehyde as an enhancer.The absence of methanol and acetonitrile in eluent made the proposed method more sensitive and eco-friendly.Under the optimal conditions,the linear range of the proposed method was in the range of 0.02-0.5μg/mL.The limit of detection（LOD） was in the range of 0.6 and 5.1μg/L.The relative standard deviations （RSD） for 0.1μg/mL mixed standard solution were in the range of 0.8-1.9%（n = 11）.The method has been applied to the determination of catecholamines in human urine successfully.Excellent spiked recoveries were achieved for catecholamines ranged from 91.2%to 112.7%.

关 键 词：CATECHOLAMINES Ion chromatography CHEMILUMINESCENCE URINE 

分 类 号：O614.812[理学—无机化学] Q422[理学—化学]