枯草芽孢杆菌G87抗菌蛋白对稻瘟病菌和稻恶苗病菌的抑制作用  

Inhibition of antifungal protein from Bacillus subtilis G87 to Magnaporthe grisea and Gibberella fujikuroi

作　　者：纪兆林[1] 李娟[1] 陈夕军[1] 童蕴慧[1] 徐敬友[1] 

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2012年第2期83-87,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(30571243);扬州大学高层次人才科研启动基金资助项目(0274983014811)

摘　　要：为明确枯草芽孢杆菌(Bacillus subtilis)G87抗菌蛋白的生物活性,采用菌丝生长、分生孢子萌发和芽管伸长抑制的方法,研究其对稻瘟病菌(Magnaporthe grisea)和稻恶苗病菌(Gibberella fujikuroi)的抑制作用。结果表明:抗菌蛋白能明显抑制2种病菌菌丝生长、分生孢子萌发和芽管伸长。1.20mg·mL-1抗菌蛋白对稻瘟病菌菌落直径和菌丝干重的抑制率分别达60%和80%以上;2.40mg·mL-1抗菌蛋白对稻恶苗病菌分生孢子萌发和芽管伸长的抑制率均达60%以上。抗菌蛋白还能显著破坏2种病菌菌丝形态,使菌丝细胞畸形膨大、原生质浓缩和外渗、细胞壁破损以及菌体崩溃等。因此,枯草芽孢杆菌G87抗菌蛋白具有重要的抗菌生物活性。In order to clarify the biological activity of antifungal protein from Bacillus subtilis G87,its inhibition to Magnaporthe grisea and Gibberella fujikuroi was studied.The results showed that the antifungal protein exhibited inhibitory activity on mycelial growth,conidial germination and germ-tube elongation of rice pathogenic fungi mentioned above.At the concentration of 1.20 mg·mL-1 for the antifungal protein,the inhibiting rates were above 60% and 80% against the colony growth and the dry mycelial weight of M.grisea,respectively.In the presence of 2.40 mg·mL-1 antifungal protein,the inhibiting rates toward the conidial germination and germ-tube elongation of G.fujikuroi were both over 60%.Moreover,the antifungal protein was demonstrated to have a destructive effect on hyphal shape of the two pathogenic fungi,with hyphal cell distortion,protoplasm concentration and leakage,tumescence,cell wall rupture,and collapse.Therefore,B.subtilis G87 antifungal protein has an important antifungal biological activity.

关 键 词：枯草芽孢杆菌 抗菌蛋白 稻瘟病菌 稻恶苗病菌 抑制作用 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]