地塞米松对大鼠烧伤合并海水浸泡后内皮祖细胞数目的影响  

Affection of Dexamethasone on the Number of Circulating Endothelial Progenitor Cells of Burned Rats with Seawater Immersion

作　　者：王培[1] 管云枫[1] 徐添颖[1] 缪朝玉[1] 

机构地区：[1]第二军医大学药学院药理学教研室,上海200433

出　　处：《西南军医》2012年第4期566-569,共4页Journal of Military Surgeon in Southwest China

基　　金：国家重点基础研究发展计划(国家科技部973计划;No.2009CB521902);国家自然科学基金(No.81100866)资助

摘　　要：目的观察早期应用地塞米松对大鼠烧伤合并海水浸泡后内皮祖细胞数目的影响。方法将52只SD大鼠随机分成6组:正常对照组,单纯烧伤组,烧伤合并海水浸泡组,单纯注射地塞米松组,烧伤+地塞米松组和烧伤合并海水浸泡+地塞米松组。在模型制备后的30分钟、2小时、6小时和24小时,使用双色荧光标记流式细胞术检测不同组别大鼠的血中的内皮祖细胞(EPC)数目。结果烧伤和海水浸泡均可显著降低血中EPC的数目。地塞米松可以部分逆转烧伤和海水浸泡对EPC数目减少的作用。结论在烧伤和海水浸泡情况下,地塞米松的使用有助于增加血EPC数目,并可能增强创面的修复能力。Objective To observe the affection of dexamethasone （DXM） on the number of circulating endothelial pro- genitor cells （EPC） of burned rats with seawater immersion. Methods 52 rats were randomly divided into 6 groups ： normal control group, DXM group, burn group, burn ＋ DXM group, burns ＋ seawater immersion group, and burns ＋ seawater immersion ＋ DXM （20 mg/kg, SC） group ; the numbers of EPC of rats in the 6 groups were detected with two - color fluorescence flow cytometry at the time point of 30 minutes, 2 hours, 6 hours and 24 hours after the model preparation. Results Burn and seawater immersion reduced the number of EPC in blood obviously, while DXM par- tially reversed this phenotype. Conclusions The application of DXM is of help in increasing the number of EPC in burned rats with seawater immersion and in enhancing the wound repair capacity of the rats.

关 键 词：烧伤 海水浸泡 地塞米松 内皮祖细胞 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]