谷酰胺对新生大鼠急性高氧肺损伤的保护机制  

Protection of Glutamine on lung injury induced by hyperoxia in neonatal rats and its mechanism

作　　者：张海鸿[1] 刘秀香[1] 赵国英[1] 王宝宏[1] 罗瑶[1] 

机构地区：[1]滨州医学院附属医院,山东滨州256603

出　　处：《山东医药》2012年第21期8-10,共3页Shandong Medical Journal

基　　金：山东省科技攻关项目(2009GG20002055);山东省优秀中青年科学家科研奖励基金(BS2010YY003);滨州医学院科研计划项目(BY2009KJ15)

摘　　要：目的探讨谷酰胺对新生大鼠急性高氧肺损伤的保护机制。方法将新生SD大鼠随机分成对照组、谷酰胺组和高氧组,比较三组大鼠存活率和体质量、肺湿质量/干质量、病理改变、热休克蛋白-70(HSP-70)和肿瘤坏死因子-α(TNF-α)的表达情况。结果高氧暴露6 d后:①高氧组体质量明显低于对照组(P<0.05),谷酰胺组与对照组比较无统计学差异(P>0.05)。②高氧组肺湿质量/干质量明显低于对照组(P<0.05),谷酰胺组与对照组比较无统计学差异(P>0.05)。③三组中高氧组肺泡炎性病理改变最为严重。④高氧组肺组织HSP-70表达量较对照组明显增加(P<0.05),谷酰胺组较对照组、高氧组均显著增加(P均<0.01);高氧组肺组织TNF-α表达量较对照组与谷酰胺组均明显增加(P均<0.01),谷酰胺组较高氧组明显减少(P<0.05)。结论谷酰胺可能通过调节炎症反应与抗炎反应之间的平衡对高氧肺损伤起到保护作用。Objective To explore the protection of glutamine on lung injury induced by hyperoxia in neonatal rats and its possible mechanism. Methods The newborn SD rats were divided into control group, glutamine group and high oxygen group at random. The expressions of right lung＇s wet weight/dry weight, heat shock protein-70 （HSP-70） and tumor nec- rosis factor-c~ （TNF-ct） of three groups newborn rats were compared. Result After exposured to high oxygen for 6 days： newborn rats＇weight and the wet/dry quality in the hyperoxia group were significantly lower than those in the control group （ all P 〈 0.05 ）. but there was no difference between the glutamine group and the control group （ P 〉 0.05 ）. Alveolar in- flammatory pathological changes in the high oxygen group were the most seriously among the three groups. The expressions of HSP-70 of lung tissue in high oxygen group were obviously increased compared with control group and that increased in glutamine group compared with control group and high oxygen group （ all P 〈 0.05 ）. The TNF-a expression of lung tissue in high oxygen group increased obviously compared with control group and glutamine group （P 〈 0.01 ） and that decreased in glutamine group compared with high oxygen group （P 〈 0.05 ）. Conclusions Glutamine may play a role in hyperoxia- induced lung injury by regulating the dynamic balance between the inflammatory and anti-inflammatory reactions.

关 键 词：高氧肺损伤 谷氨酰胺 热休克蛋白质70 肿瘤坏死因子 大鼠 

分 类 号：R563[医药卫生—呼吸系统]