MTHFR基因多态性及同型半胱氨酸与老年冠心病的相关性  

The correlation between MTHFR gene polymorphisms and levels of plasma homocysteine(Hcy) in elderly patients with coronary heart disease

作　　者：苑小历[1] 游云鹏[1] 潘士勇[1] 杨春[2] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)干部保健科,江苏南京210002 [2]南京大学医学院临床学院(南京军区南京总医院)心内科,江苏南京210002

出　　处：《东南国防医药》2012年第4期320-321,365,共3页Military Medical Journal of Southeast China

摘　　要：目的探讨N5、N10亚甲基四氢叶酸还原酶(MTHFR)基因多态性及血清同型半胱氨酸(Hcy)与老年冠心病(CHD)的相关性。方法应用聚合酶链反应限制性内切酶(RT-PCR)技术检测并比较了125例老年冠心病患者(CHD组)和60例健康老年人(对照组)的MTHFR基因型,以荧光偏振免疫分析法(FPLA)检测血清Hcy的水平并予比较。结果两组MTHFR 677位点基因型分布和各等位基因频率比较差异均有统计学意义(P均<0.05);CHD组T等位基因频率及血清Hcy水平高于对照组(P均<0.05)。结论 MTHFR基因突变可导致血清Hcy水平升高,高Hcy水平及高MTHFR基因T等位基因频率均为老年CHD的高危因素。Objective To observe the correlation of plasma Hcy and polymorphism in 5,10 MTHFR genes in the elderly patients with coronary heart disease. Methods 125 elderly patients with coronary heart dis- ease and 60 controls were studied. The Hcy levels were measured by high performance liquid chromatography fluorescence detection. The polymorphism in MTYIFR was determined by a polymerase chain reaction （PER） as- say and subsequent restriction enzyme digestion. Results The genotype distribution of MTHFR R677 site and the frequency of alleles were significant difference in CHD group and control group （P all 〈 0.05 ）. The fre- quency of T alleles and the Hcy levels were higher in the CHD group compared with those in the control group （P all 〈 0.05）. Conclusion The MTHFR gene mutations can lead to hyperhomocysteinemia. The mutations in MTHFR and hyperbomocysteinemia are both risk factors for coronary heart disease in elderly.

关 键 词：冠心病 老年人 亚甲基四氢叶酸还原酶 基因多态性 同型半胱氨酸 

分 类 号：R541.4[医药卫生—心血管疾病]