高致病性猪繁殖与呼吸综合征病毒ORF5基因的变异分析  

Variation Analysis of the ORF5 Gene of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus(HP-PRRSV)

作　　者：冷雪[1] 李真光[1] 王凤雪[1] 温永俊[1] 王炜[1] 夏铭崎 武华[1,2] 

机构地区：[1]中国农业科学院特产研究所人兽共患病研究室,特种经济动物分子生物学国家重点实验室,吉林130112 [2]华威特(北京)生物科技有限公司,北京100085

出　　处：《吉林农业大学学报》2012年第4期443-448,453,共7页Journal of Jilin Agricultural University

基　　金：科技部"十一五"科技支撑计划项目(2009BADB4B00);"863"计划项目(2011AA103A213)

摘　　要：从天津和内蒙古猪场中分离到2株高致病性猪繁殖与呼吸综合征病毒（TJ和NM1）,将TJ株经Marc-145细胞连续传代92代,得到致弱疫苗株TJM株。采用RT-PCR方法扩增TJ、NM1和TJM完整的ORF5基因片段,进行序列测定,并与其他国内外毒株的ORF5基因序列进行了比较和变异分析。结果表明：TJ株和NM1株ORF5基因与国内分离的高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV）具有较高的同源性,均为99.5%~99.8%,与欧洲型PRRSV同源性最低,分别为63.3%和63.5%。致弱疫苗株TJM与其原始毒株TJ相比有4个氨基酸发生突变（F23S,G80V,R151K,Q196R）,这些突变可能与TJ株毒力的降低有关。Two highly pathogenic porcine reproductive and respiratory syndrome virus（HP-PRRSV） isolates TJ and NM1 were obtained from Tianjin City and Neimenggu Region＇ pig farms.TJM vaccine strain was derived from HP-PRRSV TJ strain by passing 92 times in Marc-145 cells.A RT-PCR method was used to amplify the complete ORF5 gene of the viruses.Variation analysis of ORF5 gene of the three isolates and other published PRRSV isolates in or out China showed that ORF5 gene among TJ,NM1 and other HP-PRRSV had higher similarity,sharing 99.5%—99.8% identity of the nucleotide,and had the lowest similarity,sharing 63.3% or 63.5% identity of the nucleotide with the Europe LV strain.Four amino acid mutations,F23S,G80V,R151K,Q196R,occurred between TJ and TJM,and may have potential effect on the decreased virulence of TJ.

关 键 词：高致病性猪繁殖与呼吸综合征病毒 ORF5基因 变异分析 

分 类 号：S852.65[农业科学—基础兽医学]