根茬腐解液对不同豇豆品种胚根蔗糖代谢和抗氧化酶活性的影响  

Effects of the liquid of decomposed asparagus bean stubs on sucrose metabolism and antioxidant enzyme activities in hypocotyls of different asparagus bean cultivars

作　　者：刘永华[1] 杨新琴[2] 吴晓花[1] 汪宝根[1] 徐沛[1] 李国景[1] 

机构地区：[1]浙江省农业科学院蔬菜研究所,浙江杭州310021 [2]浙江省农业厅农作物管理局,浙江杭州310020

出　　处：《浙江大学学报（农业与生命科学版）》2012年第4期459-466,共8页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省自然科学基金资助项目(Y3110595);浙江省"三农五方"资助项目(SN201013)

摘　　要：利用根茬腐解液处理模拟研究自毒作用对耐自毒能力不同的2个豇豆品种胚根蔗糖代谢和抗氧化酶活性的影响。结果表明:与不耐自毒品种相比,耐自毒品种在自毒作用下具有相对较高的发芽率、液泡转化酶(VIN)、细胞质转化酶(CIN)和蔗糖合成酶(SUS)活性,以及较高的蔗糖、己糖含量和己糖/蔗糖比值;同时,耐自毒品种胚根在自毒作用下还具有相对较高的过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性和较低的丙二醛(MDA)含量。表明胚根蔗糖代谢以及CAT和SOD可能在豇豆耐自毒作用中发挥着重要作用。The effects of autotoxicity on germination rate, sucrose metabolism and antioxidant enzyme activities in hypocotyls of two asparagus bean （Vigna unguiculata ssp. sesquipedialis L. Verdc. ） cultivars, different in autotoxicity resistance, were investigated. The liquid of decomposed asparagus bean stubs was employed to simulate the autotoxicity in asparagus bean. The results showed that, compared with susceptible cultivar, resistant eultivar maintained relatively higher germination rate and activities of vacuolar invertase （VIN）, cytoplasmic invertase （CIN）, and sucrose synthase （SUS） under autotoxicity stress. At the same time, resistant cultivar also had relatively higher content of sucrose and hexose, and hexose/sucrose ratio than susceptible cultivar. Meanwhile, the resistant cultivar showed higher catalase （CAT） and superoxide dismutase （SOD） activities, and consequently lower malondialdehyde （MDA） content than the susceptible cultivar under autotoxicity stress. Therefore, it is suggested that sucrose metabolism and antioxidant enzymes may play important roles in autotoxicity resistance of asparagus bean.

关 键 词：自毒作用 蔗糖代谢 转化酶 抗氧化酶 豇豆 

分 类 号：S643.4[农业科学—蔬菜学]