1,5-二咖啡酰奎宁酸在肾上腺嗜铬细胞瘤细胞缺血再灌注损伤中的作用  

The protective effects of 1,5-diCQA on PC12 cells with OGD/reperfusion induced injury

作　　者：张莹[1,2] 付学军 卢艺[1,2] 邹良玉 曹旭[1,2] 褚晓凡 

机构地区：[1]暨南大学第二临床医学院 [2]广东省深圳市人民医院神经内科,518020

出　　处：《广东医学》2012年第14期2045-2047,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30971024);广东省自然科学基金资助项目(编号:9151051501000009);广东省深圳市科技计划项目(编号:201002004)

摘　　要：目的研究1,5-二咖啡酰奎宁酸(1,5-diCQA)对缺血再灌注诱导的大鼠肾上腺嗜铬细胞瘤细胞(PC12细胞)损伤是否有保护作用。方法用缺糖缺氧4 h/复氧24 h处理PC12细胞作为PC12细胞缺血再灌注的体外模型,实验分为4组:空白对照组、1,5-diCQA(50μmol/L)对照组、缺血再灌注组、1,5-diCQA(50μmol/L)+缺血再灌注组。应用酶标仪测定各组细胞乳酸脱氢酶(LDH)释放量,荧光显微镜观察并测定细胞内活性氧簇(ROS)含量、线粒体膜电位水平。结果 PC12细胞经缺血再灌注损伤后,与空白对照组比较,细胞LDH释放量及ROS含量显著增加(P<0.05),线粒体膜电位显著下降(P<0.05);1,5-diCQA+缺血再灌注组与缺血再灌注组比较,细胞LDH释放量及ROS含量明显降低(P<0.05),线粒体膜电位显著升高(P<0.05);1,5-diCQA对照组与空白对照组比较,以上3项指标差异无统计学意义(P>0.05)。结论 1,5-diCQA通过减轻氧化应激、保护线粒体功能,减轻缺血再灌注诱导的PC12细胞损伤。Objective To evaluate the protective effects of 1 ,5 - diCQA on PC12 cell with OGD/reperfusion - induced injury. Methods The OGD/reperfusion model was constructed with 4 hours of OGD followed with 12 hours of reoxygenation in PC12 cells in vitro. The cells were divided into 4 groups： i） blank control group, ii） 1,5 -diCQAA （50 uM） group, iii） OGD/reperfusion group, and iv） 1,5 - diCQA （50 uM） ＋ OGD/reperfusion group. The release of LDH was measured by ELISA, while the ROS content as well as MMP level were assessed by fluorescence microscope. Results Significant increase of LDH and ROS with reduction in MMP was revealed in OGD/reperfusion group when comparing with those in blank control group （P 〈 0.05 ）, which was significantly reversed by 1,5 - diCQA （50uM） treatment （P 〈 0. 05 ）. Conclusion 1,5 - diCQA can mitigate the OGD/reperfusion - induced injury to PC12 cells via alleviating oxidative stress and protecting mitochondrial function.

关 键 词：1 5-二咖啡酰奎宁酸 PC12细胞 缺血再灌注 乳酸脱氢酶 活性氧簇 线粒体膜电位 

分 类 号：R978.12[医药卫生—药品]