促孕散对持久黄体和卵巢静止奶牛血清生殖激素的影响  

A Study on the Impact of Reproductive Hormones in Serum by Using Fertility-Promoting Powder for Treating Infertility Caused by Persistent Corpus Luteum and Ovary Sandstill of Dairy Cattle

作　　者：梁光华[1] 邓敏[1] 刘贤侠[1] 谷新利[1] 高树[1] 徐雷[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003

出　　处：《中国农业科学》2012年第15期3182-3188,共7页Scientia Agricultura Sinica

基　　金："973"计划前期研究专项课题(2011CB512012);国家自然科学基金项目(31160517)

摘　　要：【目的】研究促孕散对持久黄体和卵巢静止奶牛雌二醇(E2)、促卵泡素(FSH)、促黄体素(LH)和孕酮(P4)4种生殖激素的影响,探讨促孕散治疗奶牛持久黄体和卵巢静止的作用机理。【方法】用促孕散治疗奶牛持久黄体和卵巢静止,统计发情率,用酶联免疫吸附法(ELISA)检测奶牛血清中的E2、FSH、LH和P44种生殖激素,并与正常奶牛对比。【结果】促孕散治疗持久黄体牛发情有效率为91.67%,治疗卵巢静止牛发情有效率为88.89%。促孕散处理前,持久黄体P4水平极显著高于正常组(P<0.01),卵巢静止P4极显著低于正常组(P<0.01),而两种病型在处理前E2、FSH、LH水平与正常组差异不显著(P>0.05)。持久黄体患牛在促孕散处理后血清中E2和FSH水平不断升高,与正常组比较差异不显著(P>0.05),LH水平在促孕散处理后也逐渐上升,与正常组同时达到峰值,其变化规律与正常组相似,P4水平逐渐降低,与正常组水平差异不显著(P>0.05)。卵巢静止组在促孕散处理后血清中E2、FSH和LH水平均升高,其变化规律与正常组相似,P4水平出现先升高后降低的变化,变化规律与正常组相似,差异不显著(P>0.05)。【结论】促孕散对持久黄体和卵巢静止奶牛血清生殖激素具有显著调节和诱导发情的作用。[ Objective ] In an experiment,the fertility-promoting powder was used to treat persistent corpus luteum and ovary standstill of infertility caused by ovary disease of dairy cattle,and its mechanism was studied preliminarily.【Method】 After treatment,the heat rate was counted,then the four kinds of hormones（E2,P4,FSH,LH） level were measured by ELISA in serums,and the impact of the four hormones on cows by using fertility-promoting powder was compared with normal group.【Result】The effective rate of persistent corpus luteum was 91.67% after treatment,the effective rate of ovary standstill was 88.89%.Before treatment,P4 contents were significantly higher than the normal group（P〈0.01） in persistent corpus luteum cow,but P4 contents were significantly lower than the normal group（P〈0.01） in ovary standstill cow,and the levels of E2,FSH,LH contents were not significant（P〉0.05） compared with normal group.After treatment,E2 and FSH contents continued to increase in persistent corpus luteum cow,their contents were not significantly different（P〉0.05） from the normal group.LH contents gradually increased after treatment,and peaked simultaneously with the normal group,its variation was similar to the normal group.P4 contents gradually decreased,and was not significantly different（P〉0.05） from the normal group.In ovary standstill cows,the levels of E2,FSH and LH contents increased,and the variations was similar to the normal group,the changes of P4 levels firstly increased and then decreased,its variation was similar to the normal group,and the difference was not significant（P〉0.05）.【Conclusion】 The fertility-promoting powder has a significant induction of estrus and the function of regulating reproductive hormones（E2,P4,FSH,LH） for infertility caused by ovarian disease of dairy cattle.

关 键 词：促孕散 持久黄体 卵巢静止 奶牛 生殖激素 

分 类 号：S858.23[农业科学—临床兽医学]