补肾阳方拮抗外源性糖皮质激素HPA轴抑制及分解代谢效应研究  

Effects of Kidney-yang Tonifying Formula on Inhibition of HPA Axis and Catabolism by Glucocorticoids

作　　者：曾洋[1,2] 段晓红[2] 孙伟 马方励 黄建华[2] 

机构地区：[1]上海市杨浦区安图医院,上海200093 [2]复旦大学附属华山医院中西医结合研究所,上海200040 [3]无限极(中国)有限公司,广东广州510665

出　　处：《亚太传统医药》2012年第8期5-8,共4页Asia-Pacific Traditional Medicine

基　　金：国家自然科学基金(No.30901888)

摘　　要：目的:观察补肾阳方拮抗外源性糖皮质激素HPA轴抑制及分解代谢的效果及机制。方法:将SD大鼠分为对照组,模型组,模型+补肾阳复方低、中、高剂量组。采用皮质酮注射造成肾阳虚模型。采用ELISA法检测血浆促肾上腺皮质激素(ACTH)、皮质酮浓度;取肾上腺,进行全基因组表达谱芯片检测及分析。结果:糖皮质激素造模导致大鼠体重降低,补肾阳方在第7、14天均能保护体重。补肾阳方治疗使造模组下降的肾上腺重量、ACTH、皮质酮水平升高。基因芯片结果筛选出了模型组上调或下调且能被补肾阳复方逆转的基因828个,功能分析显示涉及类固醇合成酶和细胞增殖通路。结论:补肾阳方保护糖皮质激素肾阳虚证模型的HPA轴功能,并能拮抗糖皮质激素分解效应,保护动物体重。Objective：To investigate effects and mechanisms of kidney-yang tonifying formula on inhibition of HPA axis and catabo-lism by glucocorticoids. Methods： SD rats were derided into control, model, model plus low, middle and high dose of formula group. Model was generated by injection of corticosterone. The level of plasma adrenocortieotrophin （ACTH）, eorticosterone was measured by ELISA. Whole-genome microarray analysis was conducted on adrenal gland sample. Results ： Exogenous glucocorticoids resulted in decrease in body weight gain which was protected by kidney-yang tonifying formula. The formula also increased the adre nal weight, ACTH and corticosterone level. 828 genes differentially regulated in model,and reversed by treatment group were cap-tured, which were enriched in steroid biosynthesis and cell proliferation. Conclusion：Kidney yang tonifying drugs protect the inhib-ited adrenal function, and counteract the catabolic effects of glucocorticoids.

关 键 词：肾阳虚证 基因表达 肾上腺皮质 

分 类 号：R285.5[医药卫生—中药学]