6种植物次生物质对斜纹夜蛾解毒酶活性的影响  

Effects of six plant secondary metabolites on activities of detoxification enzymes in Spodoptera litura

作　　者：王瑞龙[1,2,3] 孙玉林[1,2,3] 梁笑婷[1,2,3] 宋圆圆[1,2,3] 苏贻娟[1,2,3] 朱克岩 曾任森[1,2,3] 

机构地区：[1]华南农业大学亚热带农业生物资源保护与利用国家重点实验室,广州510642 [2]农业部华南热带农业环境重点实验室,广州510642 [3]广东省普通高等学校农业生态与农村环境重点实验室,广州510642 [4]德克萨斯农工大学昆虫学系,德克萨斯州77843美国

出　　处：《生态学报》2012年第16期5191-5198,共8页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(31000260;31100286;31028018);广东省自然科学基金项目(S2011040004336)

摘　　要：草食性昆虫取食植物时遇到宿主植物中大量次生物质的化学防御,研究昆虫适应植物毒素的反防御策略具有重要的科学意义。分别添加0.01%肉桂酸、0.01%水杨酸、0.01%花椒毒素、0.02%槲皮素、0.05%黄酮和0.1%香豆素等6种植物次生物质的人工饲料饲养斜纹夜蛾(Spodoptera litura)五龄幼虫48 h后,测定斜纹夜蛾幼虫中肠和脂肪体中谷胱甘肽S-转移酶(GSTs)、羧酸酯酶(CarE)、P450的酶含量及头部乙酰胆碱酯酶(AChE)的活性,利用半定量RT-PCR检测中肠和脂肪体中CYP4M14和CYP4S9的基因表达水平。结果表明:取食肉桂酸和香豆素后,斜纹夜蛾中肠中CarE的酶活性分别提高了1.67和1.37倍,取食6种次生物质均能显著提高斜纹夜蛾脂肪体中GSTs酶活性。取食肉桂酸和香豆素48 h后,脂肪体中P450酶含量比对照增加2.93和14.50倍。取食肉桂酸、花椒毒素、槲皮素和香豆素后,斜纹夜蛾头部AchE酶活性与对照相比提高了1.53、1.80、2.36和1.56倍。6种次生物质均可诱导脂肪体中CYP4M14基因表达,槲皮素、肉桂酸和香豆素强烈诱导CYP4S9在脂肪体中表达。表明,斜纹夜蛾具有利用植物次生物质诱导其解毒酶的能力,进而提高其对毒素的抗性。Herbivorous insects encounter chemical defense in the form of numerous secondary metabolites in host plants.Understanding insect counter-defense mechanisms is important.In this study fifth instar Spodoptera litura insects were exposed to diets containing 0.01% cinnamic acid,0.01% salicylic acid,0.01% xanthotoxin,0.02% quercetin,0.05% flavone and 0.1% coumarin for 48 h.Activities of detoxification enzymes including glutathione S-transferase（GSTs）,caboxylesterase（CarE）,cytochrome P450（P450） and acetylcholinesterase（AChE） were then determined in midguts and fatbodies,and transcript levels of CYP4M14 and CYP4S9 were examined by semi-quantitative RT-PCR.CarE activity in midguts was increased 1.67-and 1.37-fold after consumption of cinnamic acids and coumarin,respectively.All six plant secondary metabolites induced GSTs activity in the fatbodies.Total P450 activity in the fatbodies was increased 2.93-and 14.50-fold after consumption of cinnamic acid and coumarin,respectively.Consumption of cinnamic acid,xanthotoxin,quercetin and coumarin increased AChE activity 1.53-,1.80-,2.36-and 1.56-fold,respectively.All six plant secondary metabolites induced transcripts of CYP4M14 in the fatbodies of S.litura.Cinnamic acid,quercetin and coumarin strongly induced transcripts of CYP4S9 in the fatbodies.These results indicated that S.litura is able to induce its detoxification enzymes to minimize toxicity from plant secondary metabolites.

关 键 词：斜纹夜蛾 解毒酶 细胞色素P450 植物次生物质 昆虫反防御策略 

分 类 号：S482.3[农业科学—农药学]