紫茉莉愈伤组织诱导的激素优化研究  

Study on Optimization of Hormone Level in Callus Induction of Mirabilis jalapa L.

作　　者：白玉[1] 高月[1] 赵倩[1] 马慧[1] 陈丽静[1] 钟鸣[1] 

机构地区：[1]沈阳农业大学辽宁省农业生物技术重点实验室,辽宁沈阳110161

出　　处：《园艺与种苗》2012年第7期52-54,共3页Horticulture & Seed

基　　金：国家自然科学基金项目(31070448)

摘　　要：[目的]该研究以紫茉莉(Mirabilis jalapaL.)为供试植物,建立愈伤组织诱导及继代培养体系,为后期的细胞悬浮培养奠定基础。[方法]以MS为基础培养基,加入不同浓度的6-BA、2,4-D、NAA、KT,设计L16(44)正交试验。[结果]得出最佳愈伤组织诱导组合为:6-BA 0 mg/L+2,4-D 1 mg/L+NAA 1 mg/L+KT 1.5 mg/L,以附加2,4-D 1 mg/L+KT 0.5 mg/L的继代培养基上生长的愈伤组织适合细胞悬浮培养。[结论]筛选出适合紫茉莉愈伤组织培养的激素条件,可为紫茉莉组培相关研究奠定基础。[Objective] Mimbilis jalapa L. was used as the test plant to establish the system of callus induction and subculture for cell suspension culture.[Method] The experiment was designed L16,（44） orthogonal test by adding different type and coneentration of plant gruwth regulating substances,6-BA. 2.4-D.NAA,KT based on MS culture medium. [Resultl The results showed that the appropriate medium tot callus induetion is： 6-BA 0 mg/L ＋2,4-D 1 mg/L ＋ NAA 1 mg/L ＋ KT 1.5 mg/L.Based on the conclusion.the callus growing on the subculture medium with 2,4-D 1 lng/L ＋ KT 0.5 mg/L was better for （;ell suspension culture. [Conclusion] The suitable hormone level fur Mi,-at,ilis jalapa L callus induction was screened out.These could provide basis for related researches.

关 键 词：紫茉莉 愈伤组织诱导 继代培养 

分 类 号：S685.16[农业科学—观赏园艺]